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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vitro evaluation of the antioxidant activity of liposomal flavonols by the HRP--H2O2--luminol system

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Author(s):
Landi-Librandi, Ana Paula [1] ; de Oliveira, Carlos Alberto [2] ; Caleiro Seixas Azzolini, Ana Elisa [1] ; Kabeya, Luciana Mariko [1] ; Del Ciampo, Jose Orestes [3] ; Lopes Badra Bentley, Maria Vitoria [3] ; Lucisano-Valim, Yara Maria [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Dept Fis & Quim, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Fed Uberlandia, Ctr Ciencias Exatas & Tecnol, Inst Quim, BR-38400100 Uberlandia, MG - Brazil
[3] Univ Sao Paulo, Dept Ciencias Farmaceut, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Microencapsulation; v. 28, n. 4, p. 258-267, 2011.
Web of Science Citations: 11
Abstract

Considering that antioxidant flavonols have been reported to be beneficial to human health, but that their low water solubility and bioavailability limit their administration through systemic route, the development of suitable flavonol-carriers is of great importance for clinical therapeutics. The aim of this study was to prepare liposomes containing flavonols or not and evaluate their antioxidant activity. Vesicles were obtained by ethanol injection method and characterized in terms of entrapment efficiency, size and zeta potential. Inhibitory activity of liposomal flavonols on reactive oxygen species generation was assessed in vitro using luminol--H(2)O(2)--horseradish peroxidase technique. Antioxidant activity of liposomal flavonols is dependent on concentration and chemical structure of active compound. Quercetin and myricetin are the most active flavonols (IC(50) == 0.6--0.9 mu A mu mol/L), followed by kaempferol (IC(50) == 3.0--4.5 mu A mu mol/L) and galangin (IC(50) == 4.0--7.0 mu A mu mol/L). Our results suggest that antioxidant-loaded liposomes may be promising tools for therapy of diseases where oxidative stress is involved. (AU)

FAPESP's process: 07/00161-3 - Study of effect of composition of liposome containing flavonoids on Complement System activation and oxidative metabolism of human neutrophils: implications in the stability and application of this delivery system in the therapy of inflammatory diseases
Grantee:Yara Maria Lucisano Valim
Support Opportunities: Regular Research Grants
FAPESP's process: 06/04398-5 - Study of the effect of Complement System activation in the stability of different compositions of liposomes containning flavonoids: selection of the delivery system more stable and evaluation of its applicability in the therapy of inflammatory diseases.
Grantee:Ana Paula Landi Librandi
Support Opportunities: Scholarships in Brazil - Post-Doctoral