Non-ribosomal peptides produced by Brazilian cyanobacterial isolates with antimicrobial activity

Author(s)

Silva-Stenico, Maria Estela ^[1] ; Pamplona Silva, Caroline Souza ^[1] ; Lorenzi, Adriana Sturion ^[1] ; Shishido, Tania Keiko ^[1] ; Etchegaray, Augusto ^[2] ; Lira, Simone Possedente ^[3] ; Beraldo Moraes, Luiz Alberto ^[4] ; Fiore, Marli Fatima ^[1]

Affiliation

^[1] Univ Sao Paulo, Ctr Nucl Energy Agr, Mol Ecol Cyanobacteria Lab, BR-13400970 Piracicaba, SP - Brazil

^[2] Pontifical Catholic Univ Campinas, Fac Chem, BR-13012970 Campinas, SP - Brazil

^[3] Univ Sao Paulo, Dept Exact Sci, BR-13418900 Piracicaba, SP - Brazil

^[4] Univ Sao Paulo, Synthet Organ Chem Lab, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: MICROBIOLOGICAL RESEARCH; v. 166, n. 3, p. 161-175, 2011.
• Web of Science Citations: 34

Abstract

Cyanobacterial strains isolated from terrestrial and freshwater habitats in Brazil were evaluated for their antimicrobial and siderophore activities. Metabolites of fifty isolates were extracted from the supernatant culture media and cells using ethyl acetate and methanol, respectively. The extracts of 24 isolates showed antimicrobial activity against several pathogenic bacteria and one yeast. These active extracts were characterized by Q-TOF/MS. The cyanobacterial strains Cylindrospermopsis raciborskii 339-T3, Synechococcus elongatus PCC7942, Microcystis aeruginosa NPCD-1, M. panniformis SCP702 and Fischerella sp. CENA19 provided the most active extracts. The 50 cyanobacterial strains were also screened for the presence of non-ribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) genes and microcystin production. Putative fragment genes coding for NRPS adenylation domains and PKS keto-synthase domains were successfully PCR amplified from 92% and 80% of cyanobacterial strains, respectively. The potential therapeutical compounds siderophores were detected in five cyanobacterial isolates. Microcystin production was detected by ELISA test in 26% of the isolates. Further a protease inhibitor substance was detected by LC-MS/MS in the M. aeruginosa NPLJ-4 extract and the presence of aeruginosin and cyanopeptolin was confirmed by PCR amplification using specific primers, and sequenced. This screening study showed that Brazilian cyanobacterial isolates are a rich source of natural products with potential for pharmacological and biotechnological applications. (C) 2010 Elsevier GmbH. All rights reserved. (AU)

• FAPESP's process: 06/01671-2 - Molecular characterization of cyanobacteria strains isolated from São Paulo State mangrove regions
• Grantee: Caroline Souza Pamplona da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 09/13455-0 - Cyanobacteria from the caatinga biome: isolation, morphology, phylogeny and bioprospection
• Grantee: Tânia Keiko Shishido
• Support Opportunities: Scholarships in Brazil - Doctorate