A new set of microsatellite markers for the genetic characterization of Ceratocystis fimbriata, an economically important plant pathogen

Author(s)

Rizatto, Simone ^[1] ; de Araujo Batista, Carlos Eduardo ^[2] ; Bajay, Miklos Maximiliano ^[2] ; Sigrist, Mario Sergio ^[2] ; Ito, Margarida Fumiko ^[3] ; Monteiro, Mariza ^[1] ; Cavallari, Marcelo Mattos ^[1] ; Pinheiro, Jose Baldin ^[2] ; Zucchi, Maria Imaculada ^[1]

Affiliation

^[1] Polo Apta Ctr Sul, BR-13400970 Piracicaba, SP - Brazil

^[2] Univ Sao Paulo USP, Dept Genet, BR-13400970 Piracicaba, SP - Brazil

^[3] Ctr Pesquisa & Desenvolvimento Fitosanidade, Inst Agronom IAC, BR-13075630 Campinas, SP - Brazil

• Document type: Journal article
• Source: CONSERVATION GENETICS RESOURCES; v. 2, n. 1, p. 55-58, DEC 2010.
• Web of Science Citations: 3

Abstract

Ceratocystis fimbriata is a fungal pathogen which attacks several economically important plants, but occurs in host-associated, morphologically indistinguishable forms. In Brazil, this fungus seriously attacks mango trees (Mangifera indica), causing severe loss of yield. This work aimed to develop and characterize a novel set of microsatellite markers for this important pathogen, providing researchers with new molecular tools for the characterization of isolates. Twenty polymorphic primer pairs were designed from a microsatellite-enriched library. We tested the usefulness of these markers through genotyping thirteen isolates of the fungus. On average, 6.65 alleles per locus were detected, revealing the ability of this set of markers to characterize C. fimbriata isolates associated to mango and to other plant species. (AU)

• FAPESP's process: 08/02335-1 - MOLECULAR CHARACTERIZATION of Mangifera indica GERMOPLASM and the PATHOGEN Ceratocystis fimbriata
• Grantee: Simone Rizatto
• Support Opportunities: Scholarships in Brazil - Master