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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of Hepatitis B virus (HBV) genotypes in patients from Rondonia, Brazil

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Author(s):
Santos, Alcione O. [1] ; Alvarado-Mora, Monica V. [2, 3] ; Botelho, Livia [2, 3] ; Vieira, Deusilene S. [1] ; Rebello Pinho, Joao R. [2, 3] ; Carrilho, Flair J. [2, 3] ; Honda, Eduardo R. [1] ; Salcedo, Juan M. [1]
Total Authors: 8
Affiliation:
[1] CEPEM Trop Pathol Res Inst IPEPATRO, Res Ctr Trop Med, Porto Velho, RO - Brazil
[2] Univ Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Lab Gastroenterol & Hepatol, Sao Paulo Inst Trop Med, Sch Med, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: VIROLOGY JOURNAL; v. 7, NOV 12 2010.
Web of Science Citations: 38
Abstract

Background: Hepatitis B virus (HBV) can be classified into nine genotypes (A-I) defined by sequence divergence of more than 8% based on the complete genome. This study aims to identify the genotypic distribution of HBV in 40 HBsAg-positive patients from Rondonia, Brazil. A fragment of 1306 bp partially comprising surface and polymerase overlapping genes was amplified by PCR. Amplified DNA was purified and sequenced. Amplified DNA was purified and sequenced on an ABI PRISM (R) 377 Automatic Sequencer (Applied Biosystems, Foster City, CA, USA). The obtained sequences were aligned with reference sequences obtained from the GenBank using Clustal X software and then edited with Se-Al software. Phylogenetic analyses were conducted by the Markov Chain Monte Carlo (MCMC) approach using BEAST v.1.5.3. Results: The subgenotypes distribution was A1 (37.1%), D3 (22.8%), F2a (20.0%), D4 (17.1%) and D2 (2.8%). Conclusions: These results for the first HBV genotypic characterization in Rondonia state are consistent with other studies in Brazil, showing the presence of several HBV genotypes that reflects the mixed origin of the population, involving descendants from Native Americans, Europeans, and Africans. (AU)