Ex vivo assessment of genotoxicity and cytotoxicity in murine fibroblasts exposed to white MTA or white Portland cement with 15% bismuth oxide

Author(s)

Zeferino, E. G. ^[1] ; Bueno, C. E. S. ^[1] ; Oyama, L. M. ^[2] ; Ribeiro, D. A. ^[2]

Affiliation

^[1] Sao Leopoldo Mand Univ, Dent Res Ctr, Endodont Area, Campinas, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Biociencias, Sao Paulo - Brazil

• Document type: Journal article
• Source: International Endodontic Journal; v. 43, n. 10, p. 843-848, OCT 2010.
• Web of Science Citations: 19

Abstract

P>Aims To evaluate whether white mineral trioxide aggregate (MTA) or white Portland cement with 15% bismuth oxide were able to induce genetic damage and cellular death ex vivo. Methodology Aliquots of 1 x 104 murine fibroblasts were incubated at 37 degrees C for 3 h with MTA (white) or white Portland cement with 15% bismuth oxide, at final concentrations ranging from 10 to 1000 mu g mL-1 individually. Data of three independent repeats from the comet assay and the trypan blue exclusion test were assessed by the one-way anova followed by Tukey's test. Results Mineral trioxide aggregate or Portland cement containing bismuth oxide did not produce genotoxic effects with respect to the single-cell gel (comet) assay data for all concentrations evaluated. Furthermore, no cytotoxicity was observed for MTA or Portland cement. Conclusion White MTA or white Portland cement containing 15% bismuth oxide were not genotoxic and cytotoxic. (AU)

• FAPESP's process: 07/01228-4 - Medium-term oral carcinogenesis assay induced by 4-nitroquinoline 1-oxide in rats: putative biomarkers involved into its pathogenesis
• Grantee: Daniel Araki Ribeiro
• Support Opportunities: Research Grants - Young Investigators Grants