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Cell viability and bioactivity of materials based on Portland cement in primary culture cells of the follicle and dental human pulp, fibroblasts and osteoblasts

Grant number: 12/13268-9
Support type:Regular Research Grants
Duration: December 01, 2012 - November 30, 2014
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Mario Tanomaru Filho
Grantee:Mario Tanomaru Filho
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Assoc. researchers:Joni Augusto Cirelli ; Juliane Maria Guerreiro Tanomaru


Studies on primary cell cultures represent important tool for the study of the toxic effects of severous dental materials in humans. The aim of this study is to isolate human cells of the follicle and the dental pulp of third molars subjected to extraction, to characterize these cells and keep them cryopreserved for testing bioactivity and cytotoxicity of new cements. For isolation of cells it will be tested two methods: explant + enzymatic treatment with trypsin and collagenase. Once the culture system of isolated cells is obtained, the same will be characterized by the use of specific markers for osteogenesis: bone morphogenic protein 7 (BMP-7) and dentin sialoprotein (DSP); and the formation of nodules of mineralization will be evaluated by the assays Alizarin Red and alkaline phosphatase. After maintaining the established cell culture, cytotoxicity assays will be performed (MTT and Trypan Blue) with the strains isolated by comparing them with two immortalized cell lines: fibroblasts (MPDL) and osteoblastic cells (SAOS-2), using the following cements experiments: 1) Portland cement (PC) 2) White MTA (Angelus), 3) + 30% CP ONB microparticulate; 4) CP + 30% nanoparticulate ONB. (AU)

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RODRIGUES, E. M.; GOMES-CORNELIO, A. L.; SOARES-COSTA, A.; SALLES, L. P.; VELAYUTHAM, M.; ROSSA-JUNIOR, C.; GUERREIRO-TANOMARU, J. M.; TANOMARU-FILHO, M. An assessment of the overexpression of BMP-2 in transfected human osteoblast cells stimulated by mineral trioxide aggregate and Biodentine. International Endodontic Journal, v. 50, n. 2, SI, p. E9-E18, DEC 2017. Web of Science Citations: 8.
LETICIA BOLDRIN MESTIERI; ANA LÍVIA GOMES-CORNÉLIO; ELISANDRA MÁRCIA RODRIGUES; GISELE FARIA; JULIANE MARIA GUERREIRO-TANOMARU; MÁRIO TANOMARU-FILHO. Cytotoxicity and Bioactivity of Calcium Silicate Cements Combined with Niobium Oxide in Different Cell Lines. Brazilian Dental Journal, v. 28, n. 1, p. 65-71, Fev. 2017.
GOMES-CORNELIO, ANA LIVIA; RODRIGUES, ELISANDRA MARCIA; MESTIERI, LETICIA BOLDRIN; RODRIGUES SANZOVO FALCOSKI, THAIS DE OLIVEIRA; SOARES, CHRISTIANE PIENNA; GUERREIRO-TANOMARU, JULIANE MARIA; ROSSA JUNIOR, CARLOS; TANOMARU-FILHO, MARIO. Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage. Brazilian Oral Research, v. 30, n. 1 2016. Web of Science Citations: 5.
MESTIERI, LETICIA BOLDRIN; GOMES-CORNELIO, ANA LIVIA; RODRIGUES, ELISANDRA MARCIA; SALLES, LOISE PEDROSA; BOSSO-MARTELO, ROBERTA; GUERREIRO-TANOMARU, JULIANE MARIA; TANOMARU-FILHO, MARIO. Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells. Journal of Applied Oral Science, v. 23, n. 5, p. 467-471, SEP-OCT 2015. Web of Science Citations: 18.

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