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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Chlorhexidine binding to mineralized versus demineralized dentin powder

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Author(s):
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Kim, Jongryul [1] ; Uchiyama, Toshikazu [2] ; Carrilho, Marcela [3, 4] ; Agee, Kelli A. ; Mazzoni, Annalisa [5] ; Breschi, Lorenzo [6] ; Carvalho, Ricardo M. [7] ; Tjaderhane, Leo [8, 9] ; Looney, Stephen [10] ; Wimmer, Courtney [10] ; Tezvergil-Mutluay, Arzu [11] ; Tay, Franklin R. [12] ; Pashley, David H. [13]
Total Authors: 13
Affiliation:
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[1] Kyung Hee Natl Univ, Sch Dent, Dept Conservat Dent, Seoul - South Korea
[2] Nihon Univ, Sch Dent Matsuso, Dept Regenerat Dent, Chiba - Japan
[3] Univ Bandeirante Sao Paulo, Hlth Inst, GEO UNIBAN, Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Dent, Dept Dent Mat & Oral Biochem, Sao Paulo - Brazil
[5] Univ Bologna, Dept SAU & FAL, Bologna - Italy
[6] Univ Trieste IGM CNR, Dept Biomed, Unit Bologna, IOR, Bologna - Italy
[7] Univ Florida, Coll Dent, Dept Operat Dent, Gainesville, FL - USA
[8] Univ Oulu, Inst Dent, Oulu - Finland
[9] Oulu Univ Hosp, Oulu - Finland
[10] Med Coll Georgia, Dept Biostat, Augusta, GA 30912 - USA
[11] Univ Turku, Sch Dent, Dept Prosthodont, Turku - Finland
[12] Med Coll Georgia, Sch Dent, Dept Endodont, Augusta, GA 30912 - USA
[13] Med Coll Georgia, Sch Dent, Dept Oral Biol, Oral Biol Div Physiol, Augusta, GA 30912 - USA
Total Affiliations: 13
Document type: Journal article
Source: Dental Materials; v. 26, n. 8, p. 771-778, AUG 2010.
Web of Science Citations: 81
Abstract

Objectives. The purposes of this work were to quantitate the affinity and binding capacity of chlorhexidine (CHX) digluconate to mineralized versus demineralized dentin powder and to determine how much debinding would result from rinsing with water, ethanol, hydroxyethylmethacrylate (HEMA) or 0.5 M NaCl in water. Methods. Dentin powder was made from coronal dentin of extracted human third molars. Standard amounts of dentin powder were tumbled with increasing concentrations of CHX (0-30 mM) for 30 min at 37 degrees C. After centrifuging the tubes, the supernatant was removed and the decrease in CHX concentration quantitated by UV-spectroscopy. CHX-treated dentin powder was resuspended in one of the four debinding solutions for 3 min. The amount of debound CHX in the solvents was also quantitated by UV-spectroscopy. Results. As the CHX concentration in the medium increased, the CHX binding to mineralized dentin powder also increased up to 6.8 mu mol/g of dry dentin powder. Demineralized dentin powder took up significantly (p < 0.01) more CHX, reaching 30.1 mu mol CHX/g of dry dentin powder. Debinding of CHX was in the order: HEMA < ethanol < 0.05 M NaCl < water. The highest CHX binding to demineralized dentin occurred at 30 mM (1.5 wt.%). Significance. As CHX is not debound by HEMA, it may remain bound to demineralized dentin during resin-dentin bonding. This may be responsible for the long-term efficacy of CHX as an MMP inhibitor in resin-dentin bonds. (C) 2010 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved. (AU)