A column-switching method for quantification of the enantiomers of omeprazole in native matrices of waste and estuarine water samples

Author(s)

Barreiro, Juliana Cristina ^[1] ; Vanzolini, Kenia Lourenco ^[1] ; Madureira, Tania Vieira ^{[2, 3, 4]} ; Tiritan, Maria Elizabeth ^{[2, 4]} ; Cass, Quezia Bezerra ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Chem, BR-13560 Sao Carlos, SP - Brazil

^[2] Super Inst Hlth Sci N, Hlth Sci Res Ctr, Gandra, Paredes - Portugal

^[3] Univ Porto, Inst Biomed Sci Abel Salazar ICBAS, Oporto - Portugal

^[4] Univ Porto, Med Chem Ctr CEQUIMED UP, Oporto - Portugal

• Document type: Journal article
• Source: Talanta; v. 82, n. 1, p. 384-391, JUN 30 2010.
• Web of Science Citations: 29

Abstract

This work reports the use of a two-dimensional liquid chromatography (2D-LC) system for quantification of the enantiomers of omeprazole in distinct native aqueous matrices. An octyl restricted-access media bovine serum albumin column (RAM-BSA C(8)) was used in the first dimension, while a polysaccharide-based chiral column was used in the second dimension with either ultraviolet (UV-vis)or ion-trap tandem mass spectrometry (IT-MS/MS) detection. An in-line configuration was employed to assess the exclusion capacity of the RAM-BSA columns to humic substances. The excluded macromolecules had a molecular mass in the order of 18 kDa. Good selectivity, extraction efficiency, accuracy, and precision were achieved employing a very small amount (500 mu L or 1.00 mL) of native water sample per injection, with detection limits of 5.00 mu g L(-1), using UV-vis, and 0.0250 mu g L(-1), using IT-MS/MS. The total analysis time was only 35 min, with no time spent on sample preparation. The methods were successfully applied to analyze a series of waste and estuarine water samples. The enantiomers were detected in an estuarine water sample collected from the Douro River estuary (Portugal) and in an influent sample from the wastewater treatment plant (WWTP) of Sao Carlos (Brazil). As far as we are concerned, this is the first report of the occurrence of (+)-omeprazole and (-)-omeprazole in native aqueous matrices. (C) 2010 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 06/05126-9 - Enantiomeric determination of pharmaceutical drugs: omeprazole, lansoprazole and pantoprazole in urban wastewater in the city of São Carlos, SP
• Grantee: Kenia Lourenço Vanzolini
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 08/05778-1 - Chiral pharmaceuticals in wastewaters: quantification and studies of enantioselective separation mechanisms
• Grantee: Juliana Cristina Barreiro
• Support Opportunities: Scholarships in Brazil - Post-Doctoral