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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Set of virulence genes and genetic relatedness of O113: H21 Escherichia coli strains isolated from the animal reservoir and human infections in Brazil

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Author(s):
dos Santos, Luis Fernando [1] ; Irino, Kinue [2] ; Ibelli Vaz, Tania Mara [2] ; Cabilio Guth, Beatriz Ernestina [1]
Total Authors: 4
Affiliation:
[1] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil
[2] Adolfo Lutz Inst, Bacteriol Sect, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Medical Microbiology; v. 59, n. 6, p. 634-640, JUN 2010.
Web of Science Citations: 15
Abstract

Escherichia colt strains of serotype O113. H21 are commonly described as belonging to a Shiga toxin (Stx)-producing E. colt (STEC) pathotype worldwide Albeit this STEC serotype is frequently identified among cattle and other domestic animals, to the best of our knowledge no human infections associated with STEC O113. H21 have been registered in Brazil to date. Here, we report the virulence profile and genetic relatedness of a collection of O113 H21 E coli strains mainly isolated from the animal reservoir aimed at determining their potential as human pathogens. The strains from the animal reservoir (n=34) were all classified as STEC, whereas the few isolates recovered so far from human diarrhoea (n=3) lacked stx genes Among the STEC, the stX(2d-activatable) gene was identified in 85% of the strains that also carried IpfA(O113), iha, saa, ehxA, subAB, astA, cdt-V, espP, espI and epeA, the human strains harboured only lpfA(O113), iha and astA. All the strains except one, isolated from cattle, were genetically classified as phylogenetic group B1 High mass plasmids were observed in 25 isolates, but only in the STEC group were these plasmids confirmed as the STEC O113 megaplasmid (pO113) Many closely related subgroups (more than 80% similarity) were identified by PFGE, with human isolates clustering in a subgroup separate from most of the animal isolates. In conclusion, potentially pathogenic O113 H21 STEC isolates carrying virulence markers in common with O113 H21 clones associated with haemolytic uraemic syndrome cases in other regions were demonstrated to occur in the natural reservoir in our settings, and therefore the risk represented by them to public health should be carefully monitored (AU)

FAPESP's process: 05/04634-8 - Phenotypic and genotypic characterization of Escherichia coli strains of serogroup O113 isolated from human infections and from the animal reservoir
Grantee:Beatriz Ernestina Cabilio Guth
Support Opportunities: Regular Research Grants