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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Human neutrophil alloantigen-1a,-1b,-2,-3a and-4a frequencies in Brazilians

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Author(s):
Norcia, A. M. M. I. [1] ; Sugano, E. Y. K. [1] ; Chiba, A. K. [1] ; Moritz, E. [1] ; Guirao, F. P. [1] ; Yamamoto, M. [1] ; Bordin, J. O. [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo, Disciplina Hematol & Hemoterapia, BR-04023902 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: TISSUE ANTIGENS; v. 74, n. 5, p. 404-407, NOV 2009.
Web of Science Citations: 10
Abstract

Human neutrophil reactive antibodies may cause clinical disorders such as transfusion-related acute lung injury, febrile transfusion reactions, alloimmune neonatal neutropenia, immune neutropenia after stem cell transplantation, refractoriness to granulocyte transfusion, drug-induced neutropenia and autoimmune neutropenia. Using the granulocyte immunofluorescence test by flow cytometry, the phenotypic frequencies of the human neutrophil alloantigens (HNA)-1a, -1b, -2, -3a and -4a were determined in 100 healthy Brazilian persons. Neutrophils were separated from blood samples by sedimentation, centrifugated and incubated with HNA-specific alloantibody plus fluorescein isothiocyanate-labeled F(ab')(2) fragments of anti-human IgG. The results showed that the phenotype frequencies of HNA-1a, -1b, -2a, -3a and -4a were 65%, 83%, 97%, 95% and 94%, respectively. We detected that neutrophils from 17% of Brazilians typed positive only with anti-HNA-1a (HNA-1a/a), 35% only with anti-HNA-1b (HNA-1b/b) and 48% reacted with both antibodies (HNA-1a/b). The frequencies found for HNA-1a and -1b were quite similar to that reported among Africans and American-Africans, but different from those found in Japanese and Chinese. In addition, our data showed that the frequencies of HNA-2, -3a and -4a in Brazilians were comparable with those observed in Caucasians. The determination of HNAs frequencies among populations with distinct racial backgrounds is important not only for anthropological reasons, but also for neonatal typing in suspected cases of alloimmune neutropenia or when patients are severely neutropenic. (AU)

FAPESP's process: 05/55237-9 - Clinical and molecular aspects of antigens and antibodies of blood cells
Grantee:Jose Orlando Bordin
Support Opportunities: Research Projects - Thematic Grants