Schistosomicidal Evaluation of Zanthoxylum naranjillo and its Isolated Compounds against Schistosoma mansoni Adult Worms

Author(s)

Braguine, Caio G. ^[1] ; Costa, Eveline S. ^[1] ; Magalhaes, Lizandra G. ^[2] ; Rodrigues, Vanderlei ^[2] ; da Silva Filho, Ademar A. ^[2] ; Bastos, Jairo K. ^[3] ; Silva, Marcio L. A. ^[1] ; Cunha, Wilson R. ^[1] ; Januario, Ana H. ^[1] ; Pauletti, Patricia M. ^[1]

Affiliation

^[1] Univ Franca, BR-14404600 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-14040903 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Sao Paulo - Brazil

• Document type: Journal article
• Source: ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES; v. 64, n. 11-12, p. 793-797, NOV-DEC 2009.
• Web of Science Citations: 10

Abstract

Chemical investigation of the EtOAc fraction (EF) obtained from the ethanolic extract of Zanthoxylum naranjillo (Rutaceae) leaves (EE) by preparative HPLC resulted in the isolation of protocatechuic acid (1), gallic acid (2), p-hydroxybenzoic acid (3), and 5-O-caffeoylshikimic acid (4). This is the first time that the presence of compounds 1-4 in Z. naranjillo has been reported. Compounds 1-4, the EE, and EF were tested in vitro against Schistosoma mansoni adult worms. The results showed that the S. mansoni daily egg production decreased by 29.8%, 13.5% 28.4%, 17.7%, 16.3%, and 6.4%, respectively. Compounds 1 and 3 were also able to separate adult worm pairs into male and female. This activity may be correlated with the reduction in egg production, since 1 and 3 showed better inhibitory properties compared with 2 and 4. (AU)

• FAPESP's process: 08/01268-9 - Phytochemical study and evaluation of the antimalarial, antimicrobial and acetylcholinesterase inhibition activities from the São Paulo State cerrado native species
• Grantee: Ana Helena Januário
• Support Opportunities: Regular Research Grants