| Full text | |
| Author(s): |
Martins, Renato A.
[1]
;
da Silva Gomes, Guilherme A.
[1]
;
Aguiar, Jr., Odair
[1]
;
Ribeiro, Daniel A.
[1]
Total Authors: 4
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Biociencias, UNIFESP, BR-11060001 Santos, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Environment International; v. 35, n. 7, p. 1062-1065, OCT 2009. |
| Web of Science Citations: | 22 |
| Abstract | |
Owing to the influence of geno- and cytotoxicity on chemical carcinogenesis, studies have demonstrated that petroleum derivatives are able to induce genetic damage and cellular death with conflicting results so far. The aim of the present study was to comparatively evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from gas petrol attendants using two different anatomic buccal sites: cheek mucosa and lateral border of the tongue. A total of 23 gas petrol attendants and 23 health controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from cheek and lateral border of the tongue mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the above nuclear phenotypes. The results pointed out significant statistical differences (p<0.05) of micronucleated oral mucosa cells from gas petrol attendants for both oral sites evaluated. In the same way, petroleum derivate exposure was able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis, being the most pronunciated effects as those found in the lateral border of the tongue. No interaction was observed between smoking and petroleum exposure. In summary, these data indicate that gas petrol attendants comprise a high risk group for DNA damage and cellular death. It seems that the lateral border of the tongue is a more sensitive site to geno- and cytotoxic insult induced by petroleum derivates. (C) 2009 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 07/01228-4 - Medium-term oral carcinogenesis assay induced by 4-nitroquinoline 1-oxide in rats: putative biomarkers involved into its pathogenesis |
| Grantee: | Daniel Araki Ribeiro |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 07/00345-7 - DNA damage induced by metal ions from orthodontic therapy: in vivo and in vitro analyses |
| Grantee: | Daniel Araki Ribeiro |
| Support Opportunities: | Regular Research Grants |