Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular Characterization of Brazilian Infectious Bursal Disease Virus Isolated from 1997 to 2005

Full text
Author(s):
Fernandes, Maria Judite B. [1] ; Simoni, Isabela C. [1] ; Vogel, Maria Gabriela [1] ; Harakava, Ricardo [1] ; Rivas, Eliana B. [1] ; Oliveira, Maria Beatriz C. [2] ; Kanashiro, Ana M. I. [1] ; Tessari, Eliana N. C. [1] ; Gama, Nilce M. S. Q. [1] ; Arns, Clarice W. [3]
Total Authors: 10
Affiliation:
[1] Inst Biol, Sao Paulo - Brazil
[2] Lohmann Anim Hlth, BR-05412001 Rua Joao Moura, SP - Brazil
[3] Univ Estadual Campinas, Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: AVIAN DISEASES; v. 53, n. 3, p. 449-454, SEP 2009.
Web of Science Citations: 8
Abstract

This retrospective study concerned 41 infectious bursal disease virus (IBDV) isolates obtained from Brazilian broiler and layers flocks by reverse transcription-polymerase chain reaction. Twenty-five of them were identified as very virulent (vv) by restriction enzyme analysis and by further nucleotide and phylogenetic analysis. All of them had the typical amino acid residues, and all clustered in a phylogenetic tree with the vvIBDV strains. Four amino acid substitutions, at positions D213N, G254D, S317R, and D323E, were common to 3 vv isolates, Br/03/DB, Br/03/CK, and Br/04/CR, and differed from other vv isolates and strains. These isolates came from the same locale, but were collected in different years, indicating that the vvIBDVs circulating on Brazilian farms are undergoing slight but continuous exchanges. (AU)