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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dual gene transfer of fibroblast growth factor-2 and platelet derived growth factor-BB using plasmid deoxyribonucleic acid promotes effective angiogenesis and arteriogenesis in a rodent model of hindlimb ischemia

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Author(s):
De Paula, Erich V. [1] ; Flores-Nascimento, Mariane Cristina [2] ; Arruda, Valder R. [2] ; Garcia, Rosana A. [2] ; Ramos, Celso D. [2] ; Guillaumon, Ana T. [2] ; Annichino-Bizzacchi, Joyce M. [2]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas, Hematol & Hemotherapy Ctr, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Fac Med Sci, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: TRANSLATIONAL RESEARCH; v. 153, n. 5, p. 232-239, MAY 2009.
Web of Science Citations: 8
Abstract

The protein infusion of basic fibroblast growth factor-2 (FGF-2) and platelet derived growth factor-BB (PDGF-BB) have been shown to promote the formation of a stable and functional vascular network in small and large animal models of ischemia. Here, we sought to determine whether a similar effect could be obtained using a gene-therapy-based strategy with nonviral vectors. Rats underwent a surgical procedure to create hindlimb ischemia and were injected with a combination of plasmids that expressed FGF-2 and PDGF-BB. Anatomical and functional parameters of the angiogenesis and arteriogenesis response were evaluated after 4 weeks. The results were compared with rats injected with plasmids that expressed a reporter gene or the extensively studied vascular endothelial growth factor (VEGF165) alone. Treatment with the FGF-2/PDGF-BB combination increased the angiogenesis and arteriogenesis response compared with the empty plasmid, and it was as effective as VEGF165. In terms of safety, the combination allowed the use of a 50% lower individual dose of each plasmid and in addition promoted the formation of more stable vessels than VEGF165. In conclusion, the dual gene transfer of FGF-2 and PDGF-BB using nonviral vectors is safe and effective in promoting the formation of a functional vascular network in a rodent model of hindlimb ischemia. (Translational Research 2009; 153:232-239) (AU)

FAPESP's process: 04/13158-2 - Arterial and venous vascular disease: physiopathology, identification of niew risk factors, and gene therapy
Grantee:Joyce Maria Annichino-Bizzacchi
Support Opportunities: Research Projects - Thematic Grants