Arterial and venous vascular disease: physiopathology, identification of niew risk factors, and gene therapy

• Grant number: 04/13158-2
• Support Opportunities: Research Projects - Thematic Grants
• Start date: March 01, 2005
• End date: December 31, 2008
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Joyce Maria Annichino-Bizzacchi
• Grantee: Joyce Maria Annichino-Bizzacchi
• Host Institution: Centro de Hematologia e Hemoterapia (HEMOCENTRO). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Pesquisadores principais: Nelci Fenalti Höehr

Abstract

In this project we propose an evaluation of coagulation and homocysteine in the kinetics and extension of the histopathological lesions of arteriosclerosis, using murine models deficient of apoE, of LDL or of FVIII or FIX receptors of coagulation. Hyperhomocysteinemia will be induced by specific diet. We will evaluate the effectiveness of gene therapy in the induction of angiogenesis in model with peripheral arterial ischemia in mice, derived from the local expression of the gene of the VEGF and the Ang1, through the use or viral and non viral vectors, in vivo. Another objective is to evaluate the effect of anticoagulant therapy in the formation of new vessels induced by gene therapy. To identify new risk factors in DVT we will analyze the genic expression of inflammatory mediators in the mononuclear cells of patients with DVT through the technique of cDNA-microarray. We will also evaluate the role of the platelets in the physiopathology and evolution of DVT by the detection of platelet microparticles in patients with DVT (acute and chronic phase) and in asymptomatic carriers of thrombophilia. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE PAULA, ERICH V.; FLORES-NASCIMENTO, MARIANE CRISTINA; ARRUDA, VALDER R.; GARCIA, ROSANA A.; RAMOS, CELSO D.; GUILLAUMON, ANA T.; ANNICHINO-BIZZACCHI, JOYCE M.. Dual gene transfer of fibroblast growth factor-2 and platelet derived growth factor-BB using plasmid deoxyribonucleic acid promotes effective angiogenesis and arteriogenesis in a rodent model of hindlimb ischemia. TRANSLATIONAL RESEARCH, v. 153, n. 5, p. 232-239, MAY 2009. (04/13158-2)