The involvement of RPA complex and ORC1 protein in the replication of chromossomal...
| Full text | |
| Author(s): |
de Oliveir, Juliana Ferreira
[1]
;
Castilho, Beatriz A.
[2]
;
Sforca, Mauricio L.
[1]
;
Krieger, Marco Aurelio
[3]
;
Zeri, Ana Carolina
[1]
;
Guimaraes, Beatriz G.
[1]
;
Zanchin, Nilson I. T.
[1]
Total Authors: 7
|
| Affiliation: | [1] LNLS, Brazilian Synchrotron Light Lab, Ctr Struct Mol Biol, BR-13083970 Campinas, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 Sao Paulo - Brazil
[3] IBMP, Curitiba, Parana - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Biochimie; v. 91, n. 4, p. 475-483, APR 2009. |
| Web of Science Citations: | 2 |
| Abstract | |
The human SBDS gene and its yeast ortholog SDOI encode essential proteins that are involved in ribosome biosynthesis. SDOI has been implicated in recycling of the ribosomal biogenesis factor Tif6p from pre-66S particles as well as in translation activation of 60S ribosomes. The SBDS protein is highly conserved, containing approximately 250 amino acid residues in animals, fungi and Archaea, while SBDS orthologs of plants and a group of protists contain an extended C-terminal region. In this work, we describe the characterization of the Trypanosoma cruzi SBDS ortholog (TcSBDS). TcSBDS co-fractionates with polysomes in sucrose density gradients, which is consistent with a role in ribosome biosynthesis. We show that TcSBDS contains a C-terminal extension of 200 amino acids that displays the features of intrinsically disordered proteins as determined by proteolytic, circular dichroism and NMR analyses. Interestingly, the C-terminal extension is responsible for TcSBDS-RNA interaction activity in electrophoretic mobility shift assays. This finding suggests that Trypanosomatidae and possibly also other organisms containing SBDS with extended C-terminal regions have evolved an additional function for SBDS in ribosome biogenesis. (C) 2008 Elsevier Masson SAS. All rights reserved. (AU) | |
| FAPESP's process: | 00/10266-8 - A structural biology laboratory network for the study of the 3D structures of proteins |
| Grantee: | Nilson Ivo Tonin Zanchin |
| Support type: | Genome Research Grants |
| FAPESP's process: | 98/14138-2 - Center for Structural Molecular Biotechnology |
| Grantee: | Glaucius Oliva |
| Support type: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 02/12597-7 - Protein synthesis in eukaryotes |
| Grantee: | Beatriz Amaral de Castilho |
| Support type: | Research Projects - Thematic Grants |