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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mineral distribution and CLSM analysis of secondary caries inhibition by fluoride/MDPB-containing adhesive system after cariogenic challenges

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Author(s):
Galbiatti de Carvalho, Fabiola [1] ; Puppin-Rontani, Regina M. [1, 2] ; Soares, Luis E. S. [3, 4] ; Santo, Ana Maria E. [4] ; Martin, Airton A. [4] ; Nociti-Junior, Francisco H. [5]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, Fac Dent Piracicaba, Dent Mat Area, Dept Restorat Dent, Sao Paulo - Brazil
[2] Univ Estadual Campinas, Fac Dent Piracicaba, Dept Pediat Dent, Sao Paulo - Brazil
[3] Vale Paraiba Univ, UNIVAP, Fac Dent, Dept Dent Mat & Operat Dent, BR-12244000 Sao Paulo - Brazil
[4] Vale Paraiba Univ, UNIVAP, Inst Res & Dev, Lab Biomed Vibrat Spect, LEVB, IP&D, BR-12244000 Sao Paulo - Brazil
[5] Univ Estadual Campinas, Fac Dent Piracicaba, Periodont Area, Dept Prosthodont & Periodont, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Journal of Dentistry; v. 37, n. 4, p. 307-314, APR 2009.
Web of Science Citations: 27
Abstract

Objective: To evaluate the inhibition zone formation (IZ) and mineral distribution along the interface of adhesive systems either containing fluoride and antibacterial primer or not, after chemical and biological artificial caries challenges. Methods: Forty-eight third molars were used. Artificial caries was developed with S. mutans in a 4 mm x 4 mm area of occlusal dentin surface. Carious dentin was removed and cavities were restored with Adper Scotchbond Multi-Purpose (SBM) and Clearfil Protect Bond (CPB) (n = 24). Samples were submitted to secondary caries development by chemical (C) (acidic gel) or biological (B) (S. mutans culture) methods for 5 days. Four groups were tested (n = 12): (1) SC (SBM + C); (2) SB (SBM + B); (3) CC (CPB + C); (4) CB (CPB + B). The IZ and outer lesion (OL) formations were analyzed by confocal. laser scanning microscopy (CLSM). The distribution of calcium (Ca) and phosphorus (P) content along the interface was analyzed by micro X-ray fluorescence spectrometer by energy-dispersive (mu EDX). Results: The frequency of IZ formation and mean values of IZ thickness differed among the groups. The CC group presented the lowest OL depth. mu EDX analysis showed that CPB had the highest mineral loss by the biological method, but the lowest mineral loss by the chemical method. SC and SB groups showed intermediate values of mineral loss. Conclusion: The mineral loss along the dentin/restoration inter-face was affected by the artificial caries method, and hybrid layer formation by adhesive systems used. The adhesive system containing fluoride and antibacterial primer did not prevent secondary caries formation. (C) 2008 Elsevier Ltd. All rights reserved. (AU)