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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Chlorhexidine Inhibits the Proteolytic Activity of Root and Coronal Carious Dentin in vitro

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Author(s):
Garcia, M. B. [1, 2] ; Carrilho, M. R. [3, 4] ; Nor, J. E. [5] ; Anauate-Netto, C. [3] ; Anido-Anido, A. [3] ; Amore, R. [3] ; Tjaderhane, L. [6, 7] ; Bretz, W. A. [8]
Total Authors: 8
Affiliation:
[1] Univ Sao Francisco, Sch Dent, Dept Biochem, BR-12900000 Braganca Paulista, SP - Brazil
[2] Univ Sao Francisco, Sch Pharm, BR-12900000 Braganca Paulista, SP - Brazil
[3] Univ Bandeirante Sao Paulo, Sch Dent, Sao Paulo - Brazil
[4] Univ Estadual Campinas, Dept Restorat Dent, Sch Dent, Piracicaba - Brazil
[5] Univ Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 - USA
[6] Univ Oulu, Inst Dent, Oulu - Finland
[7] Oulu Univ Hosp, Oulu - Finland
[8] New York Univ, Coll Dent, Dept Cariol & Comprehens Care, New York, NY 10010 - USA
Total Affiliations: 8
Document type: Journal article
Source: Caries Research; v. 43, n. 2, p. 92-96, 2009.
Web of Science Citations: 13
Abstract

The purpose of this study was to evaluate the effect of chlorhexidine on the proteolytic activity of carious coronal and root dentin collected from patients. Sound dentin from freshly extracted human teeth was used as a control. Dentin fragments were mixed with a synthetic substrate for proteolytic enzymes (N-benzoyl-DL-arginine-naphthylamide BANA) and the suspensions mixed with either 0.12% chlorhexidine digluconate or distilled water. These mixtures were incubated for 18 h at 37 degrees C, color was developed by the addition of 0.1% Fast Garnet and their optical density was recorded spectrophotometrically. BANA hydrolysis measured by the optical density of incubated specimens was detected in all tested groups, but was significantly higher for carious than for sound dentin (p < 0.05). The proteolytic activity was reduced for carious coronal and root dentin by chlorhexidine (p < 0.05; 50 and 30%, respectively). Chlorhexidine also reduced the proteolytic activity in sound root dentin (p < 0.05; 20%). Conversely, changes in the proteolytic activity of sound coronal dentin were not observed in the presence of chlorhexidine. The reduction in proteolytic activity by chlorhexidine was significantly higher in carious coronal dentin than in carious root dentin (p < 0.05). In conclusion, part of the effect of chlorhexidine in controlling caries progression in humans may be due to a decrease in the proteolytic activity of carious coronal and root dentin. Because of the prolonged incubation time in the present study, similar results may be obtained clinically with prolonged dentin exposure to chlorhexidine, e. g. chlorhexidine-containing varnishes. Copyright (C) 2009 S. Karger AG, Basel (AU)