Co-ordinated expression of lymphoid and myeloid specific transcription factors during B-1b cell differentiation into mononuclear phagocytes in vitro

Author(s)

Popi, Ana F. ^[1] ; Motta, Fabiana L. T. ^[2] ; Mortara, Renato A. ^[1] ; Schenkman, Sergio ^[1] ; Lopes, Jose D. ^[1] ; Mariano, Mario ^[1]

Affiliation

^[1] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, CEDEME Ctr Desenvolvimento Modelos Expt Med & Bio, Sao Paulo - Brazil

• Document type: Journal article
• Source: IMMUNOLOGY; v. 126, n. 1, p. 114-122, JAN 2009.
• Web of Science Citations: 34

Abstract

We previously demonstrated that B-1b cells can undergo differentiation to acquire a mononuclear phagocyte phenotype upon attachment to substrate in vitro. Here we followed the expression of surface markers and transcription factors during this differentiation. B-1b cells spontaneously express both myeloid and lymphoid restricted transcription factors. When induced to differentiate into a phagocyte, the lymphoid genes E box protein (E2A), early B-cell factor (EBF), paired box 5 (Pax5) are down-modulated, while expression of genes related to myeloid commitment is sustained. Furthermore, B-1b cell-derived phagocytes (B-1CDPs) decrease immunoglobulin M (IgM) expression but retain the expression of the heavy chain variable gene VH11 or VH12, an immunoglobulin gene rearrangement predominantly expressed by B-1 cells. The maintenance of lymphoid characteristics in B-1CDPs characterizes a unique type of phagocyte, not related to monocyte-derived macrophages. (AU)

• FAPESP's process: 04/08506-1 - B-1 cells: origin, differentiation and participation in inflammation, scarring and rejection of implant
• Grantee: Mario Mariano
• Support Opportunities: Research Projects - Thematic Grants