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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Amitriptyline and Acute Inflammation: A Study Using Intravital Microscopy and the Carrageenan-Induced Paw Edema Model

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Author(s):
Vismari, L. ; Alves, G. J. ; Palermo-Neto, J. [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Lab Farmacol Aplicada & Toxicol, Neuroimmunomodulat Res Grp, Dept Pathol, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Pharmacology; v. 86, n. 4, p. 231-239, 2010.
Web of Science Citations: 9
Abstract

Background/Aims: Antidepressants are reported to exhibit antiinflammatory effects. However, mechanisms involved in this action have not been elucidated. Thus, the objectives of the present study were (a) to evaluate the effects of amitriptyline on the acute inflammatory process, and (b) to investigate the participation of alpha(1)-adrenergic receptors and glucocorticoids as possible mechanisms implicated in the amitriptyline action on inflammation. Methods and Results: Single and multiple doses of amitriptyline were administered to rats submitted to the carrageenan-induced paw edema model. The results showed a significant antiedematous reaction to amitriptyline, mainly when administered at each elimination half-life. The next step was to evaluate its effects on leukocyte behavior, using intravital microscopy. Amitriptyline produced a significant effect on leukocyte behavior. To investigate possible mechanisms involved, a glucocorticoid receptor antagonist (RU-486) and an alpha(1)-adrenergic receptor antagonist (prazosin) were used. RU-486 administration lacked the ability to decrease the amitriptyline antiinflammatory effects in the carrageenan-induced paw edema model. Prazosin pretreatment potentiated the amitriptyline antiinflammatory effect without presenting an effect per se. Conclusion:The present study shows the ability of amitriptyline to decrease edema and affect leukocyte behavior in an acute inflammatory process; and, for the first time to our knowledge, we suggest the involvement of alpha(1)-adrenoceptors in the antiinflammatory effects of amitriptyline. Copyright (C) 2010 S. Karger AG, Basel (AU)

FAPESP's process: 04/14128-0 - Neuroimmunomodulation: effects of drugs, stress and cytocines on central nervous and immune systems bidirectional relationships
Grantee:João Palermo Neto
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 99/04228-7 - Neuroimmunomodulation: effects of stress and central nervous system acting drugs on immune/inflamatory response
Grantee:João Palermo Neto
Support Opportunities: Research Projects - Thematic Grants