| Full text | |
| Author(s): |
Svensjö‚ E.
;
Boschcov‚ P.
;
Ketelhuth‚ DFJ
;
Jancar‚ S.
;
Gidlund‚ M.
Total Authors: 5
|
| Document type: | Journal article |
| Source: | Inflammation Research; v. 52, n. 5, p. 215-220, 2003. |
| Abstract | |
Objective and Design: Oxidized low-density lipoproteins (oxLDL) and protein fractions obtained by size exclusion chromatography of oxLDL were tested for vascular permeability effects on topical application to the hamster cheek pouch. Materials: The hamster cheek pouch was prepared for intravital microscopy observations of macromolecular leakage at post capillary venules (= leaks) with FITC-dextran as tracer. Treatment: OxLDL (0.1 mg/ml), PAF (platelet activation factor, 50-100 nM) and protein fractions of oxLDL (10 mug/ml) were applied topically to hamster cheek pouches. Results: Application of oxLDL and PAF resulted in reversible increases in the number of leaks. The PAF-antagonist WEB 2170, L-NAME and a beta(2)-adrenoceptor agonist inhibited (P < 0.01) almost completely the macromolecular leakage induced with oxLDL or PAF. Protein fractions were found to be more effective than unfractionated oxLDL in inducing plasma leakage as calculated on mg/ml-basis. Conclusion: Hamster oxLDL is a potent inducer of macromolecular leakage increase in the hamster cheek pouch microcirculation. The principal effect is mediated by PAF-like structures produced by the oxidation of the LDL-particle but oxLDL also contains low molecular weight proteins that could contribute to the overall vascular permeability increasing effect of ox LDL. (AU) | |
| FAPESP's process: | 00/05327-8 - Pathological consequences of leukocytes activation: modulation by lipid mediators |
| Grantee: | Sônia Jancar |
| Support Opportunities: | Research Projects - Thematic Grants |