Antimicrobial Evaluation of Diterpenes from Copaifera langsdorffii Oleoresin Against Periodontal Anaerobic Bacteria

Author(s):Show less -

Souza, Ariana B. ^[1] ; de Souza, Maria G. M. ^[1] ; Moreira, Maisa A. ^[1] ; Moreira, Monique R. ^[1] ; Furtado, Niege A. J. C. ^[2] ; Martins, Carlos H. G. ^[1] ; Bastos, Jairo K. ^[2] ; dos Santos, Raquel A. ^[1] ; Heleno, Vladimir C. G. ^[1] ; Ambrosio, Sergio Ricardo ^[1] ; Veneziani, Rodrigo C. S. ^[1]

Affiliation

^[1] Univ Franca, BR-14404600 Franca, SP - Brazil

^[2] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Molecules; v. 16, n. 11, p. 9611-9619, 2011.
• Web of Science Citations: 53

Abstract

The antimicrobial activity of four labdane-type diterpenes isolated from the oleoresin of Copaifera langsdorffii as well as of two commercially available diterpenes (sclareol and manool) was investigated against a representative panel of microorganisms responsible for periodontitis. Among all the evaluated compounds, (-)-copalic acid (CA) was the most active, displaying a very promising MIC value (3.1 mu g mL(-1); 10.2 mu M) against the key pathogen (Porphyromonas gingivalis) involved in this infectious disease. Moreover, CA did not exhibit cytotoxicity when tested in human fibroblasts. Time-kill curve assays performed with CA against P. gingivalis revealed that this compound only inhibited the growth of the inoculums in the first 12 h (bacteriostatic effect). However, its bactericidal effect was clearly noted thereafter (between 12 and 24 h). It was also possible to verify an additive effect when CA and chlorhexidine dihydrochloride (CHD, positive control) were associated at their MBC values. The time curve profile resulting from this combination showed that this association needed only six hours for the bactericidal effect to be noted. In summary, CA has shown to be an important metabolite for the control of periodontal diseases. Moreover, the use of standardized extracts based on copaiba oleoresin with high CA contents can be an important strategy in the development of novel oral care products. (AU)

• FAPESP's process: 09/12796-9 - Copaifera langsdorfii oleoresin: isolation of secondary metabolites, development and validation of analytical methodology by HPLC
• Grantee: Ariana Borges Souza
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 09/09438-3 - Copaifera langsdorfii oleoresin: isolation of secondary metabolites, evaluation of their antimicrobial potential against oral pathogens and development and validation of analytical methodology by HPLC.
• Grantee: Rodrigo Cassio Sola Veneziani
• Support Opportunities: Regular Research Grants