Response surface modelling of the production of structured lipids from soybean oil using Rhizomucor miehei lipase

Author(s)

Melo Branco de Araujo, Maria Elisa ^[1] ; Bueno Campos, Paula Renata ^[1] ; Noso, Tatiana Mikie ^[1] ; Alberici, Rosana Maria ^[2] ; da Silva Cunha, Ildenize Barbosa ^[2] ; Simas, Rosineide Costa ^[2] ; Eberlin, Marcos Nogueira ^[2] ; Carvalho, Patricia de Oliveira ^[1]

Affiliation

^[1] Univ Sao Francisco, BR-12916900 Braganca Paulista, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem, ThoMSon Mass Spectrometry Lab, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: Food Chemistry; v. 127, n. 1, p. 28-33, 2011.
• Web of Science Citations: 15

Abstract

The production of structured lipids (SLs) by the acidolysis of soybean oil (SO) with a free fatty acid (FFA) mixture obtained from Brazilian sardine oil, catalysed by Rhizomucor miehei lipase (Lipozyme RM IM) in a solvent-free medium, was optimised by response surface methodology (RSM) using a three-factor central composite rotatable design. The best reaction conditions to achieve an adequate n-6/n-3 FA ratio were: sardine-FFA:SO mole ratio of 3:1, initial water content of the enzyme of 0.87% w/w, reaction time of 12 h, reaction temperature of 40 C and 10% by weight of the enzyme (% w/w). Under these conditions, the incorporation of eicosapentaenoic acid (EPA) + docosahexaenoic acid (DHA) into the soybean oil reached 9.2% (% of the total FAs), leading to a significant reduction in the n-6/n-3 FA ratio from 11:1 to 3:1. Analysis of variance (ANOVA) showed that 95% (R-2 = 0.95) of the observed variation was explained by the model. Lack of fit analysis revealed a non-significant value for the model equation, indicating that the regression equation was adequate for predicting the degree of EPA + DHA incorporation under any combination of values of the variables. Easy ambient sonic-spray ionisation mass spectrometry (EASI-MS) was used for instantaneous characterisation of TAGs. After the enzymatic reaction, a great variety of new TAGs were formed containing EPA, DHA or both in the same molecule. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 08/01235-3 - Biocatalysis in health: obtaining biological active lipids by immobilized lipases
• Grantee: Patrícia de Oliveira Carvalho
• Support Opportunities: Regular Research Grants