| Full text | |
| Author(s): |
Cavalcante‚ A.K.D.
;
Martinez‚ G.R.
;
Di Mascio‚ P.
;
Menck‚ C.F.M.
;
Agnez-Lima‚ L.F.
Total Authors: 5
|
| Document type: | Journal article |
| Source: | DNA Repair; v. 1, n. 12, p. 1051-1056, 2002. |
| Abstract | |
Singlet oxygen (O-1(2)) is a product of several biological processes and can be generated in photodynamic therapy, through a photosensitization type H mechanism. O-1(2) is able to interact with lipids, proteins and DNA, leading to cell killing and mutagenesis, and can be directly involved with degenerative processes such as cancer and aging. In this work, we analyzed the cytotoxicity and mutagenesis induced after direct treatment of wild type and the DNA repair fpg and/or mutY deficient Escherichia coli strains with disodium 3,3'-(1,4-naphthylidene) diproprionate endoperoxide (NDPO2), which releases O-1(2) by thermodissociation. The treatment induced cell killing and mutagenesis in all strains, but the mutY strain showed to be more sensitive. These results indicate that even O-1(2) generated outside bacterial cells may lead to DNA damage that could be repaired by pathways that employ MutY protein. As O-1(2) is highly reactive, its interaction with cell membranes may generate secondary products that could react with DNA, leading to mutagenic lesions. (C) 2002 Elsevier Science B.V. All rights reserved. (AU) | |
| FAPESP's process: | 00/03878-7 - Characterization of biomolecules modified by oxidative mechanisms, especially via singlet oxygen, in chemical and biological systems |
| Grantee: | Paolo Di Mascio |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 98/11119-7 - Repair of damaged DNA and biological consequences |
| Grantee: | Carlos Frederico Martins Menck |
| Support Opportunities: | Research Projects - Thematic Grants |