| Full text | |
| Author(s): |
Chiganças‚ V.
;
Miyaji‚ E.N.
;
Muotri‚ A.R.
;
Jacysyn‚ J.F.
;
Amarante-Mendes‚ G.P.
;
Yasui‚ A.
;
Menck‚ C.F.M.
Total Authors: 7
|
| Document type: | Journal article |
| Source: | Cancer Research; v. 60, n. 9, p. 2458, 2000. |
| Abstract | |
Photolyase absorbs blue light and employs the energy to remove UV-induced DNA damage, cyclobutane pyrimidine dimers, or pyrimidine pyrimidone (6-4) Lesions. These enzymes have been found in many living organisms ranging from bacteria to aplacental mammals, but their photoreactivation effect, such as survival increase of W-irradiated cells by light-illumination, has not been identified in placental mammals, including humans. Therefore, we introduced a photolyase gene derived from the marsupial rat kangaroo, Potorous tridactylus, into HeLa cells and established the first human cell line capable of photorepairing UV-induced pyrimidine dimers, Several clones were found to increase cell survival after UV irradiation when illuminated by fluorescent light. The induction of apoptosis by UV irradiation was investigated in these photoreactivation-proficient cells. Several typical features of the programmed cell death, such as internucleosomal DNA degradation, presence of subdiploid cells, loss of membrane integrity, and chromosomal condensation, were found to be induced by UV in the HeLa cells, but they can be reduced by photorepair, This implicates that cyclobutane pyrimidine dimers cause W-induced apoptosis in human cells. (AU) | |
| FAPESP's process: | 96/04518-7 - Studies of DNA repair and its biological consequences |
| Grantee: | Carlos Frederico Martins Menck |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 98/11119-7 - Repair of damaged DNA and biological consequences |
| Grantee: | Carlos Frederico Martins Menck |
| Support Opportunities: | Research Projects - Thematic Grants |