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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of immobilized lipases on poly-hydroxybutyrate beads to catalyze biodiesel synthesis

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Author(s):
Mendes, Adriano A. [1, 2] ; Oliveira, Pedro C. [3] ; Velez, Ana M. [1] ; Giordano, Roberto C. [1] ; Giordano, Raquel de L. C. [1] ; de Castro, Heizir F. [3]
Total Authors: 6
Affiliation:
[1] Univ Fed Sao Carlos, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Joao del Rei, Lab Biocatalysis, BR-35701970 Sete Lagoas, MG - Brazil
[3] Univ Sao Paulo, Dept Chem Engn, Engn Sch Lorena, BR-12602810 Lorena, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: International Journal of Biological Macromolecules; v. 50, n. 3, p. 503-511, APR 1 2012.
Web of Science Citations: 49
Abstract

Five microbial lipase preparations from several sources were immobilized by hydrophobic adsorption on small or large poly-hydroxybutyrate (PHB) beads and the effect of the support particle size on the biocatalyst activity was assessed in the hydrolysis of olive oil, esterification of butyric acid with butanol and transesterification of babassu oil (Orbignya sp.) with ethanol. The catalytic activity of the immobilized lipases in both olive oil hydrolysis and biodiesel synthesis was influenced by the particle size of PHB and lipase source. In the esterification reaction such influence was not observed. Geobacillus thermocatenulatus lipase (BTL2) was considered to be inadequate to catalyze biodiesel synthesis, but displayed high esterification activity. Butyl butyrate synthesis catalyzed by BTL2 immobilized on small PHB beads gave the highest yield (approximate to 90 mmol L-1). In biodiesel synthesis, the catalytic activity of the immobilized lipases was significantly increased in comparison to the free lipases. Full conversion of babassu oil into ethyl esters was achieved at 72 h in the presence of Pseudozyma antarctica type B (CALB), Thermomyces lanuginosus lipase (Lipex (R) 100L) immobilized on either small or large PHB beads and Pseudomonas fluorescens (PFL) immobilized on large PHB beads. The latter preparation presented the highest productivity (40.9 mg of ethyl esters mg(-1) immobilized protein h(-1)). (C) 2012 Elsevier B.V. All rights reserved. (AU)