Separation of clavulanic acid from fermented broth of amino acids by an aqueous two-phase system and ion-exchange adsorption

Author(s)

da Silva, Clovis Sacardo ^[1] ; Cuel, Maressa Fabiano ^[1] ; Barreto, Veronica Orlandin ^[1] ; Kwong, Wu Hong ^[1] ; Hokka, Carlos O. ^[1] ; Barboza, M. ^{[1, 2]}

Affiliation

^[1] Univ Fed Sao Carlos, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Triangulo Mineiro, Dept Chem Engn, Inst Ciencias Tecnol & Exatas, Univerdicidade Uberaba, MG - Brazil

• Document type: Journal article
• Source: NEW BIOTECHNOLOGY; v. 29, n. 3, p. 428-431, FEB 15 2012.
• Web of Science Citations: 8

Abstract

The clavulanic acid is a substance which inhibits the beta-lactamases used with penicillins for therapeutic treatment. After the fermentation, by-products of low molecular weight such as amino acids lysine, histidine, proline and tyrosine are present in the fermented broth. To remove these impurities the techniques of extraction by an aqueous two-phase system of 17% polyethylene glycol molecular weight 600 and 15% potassium phosphate were used for a partial purification. A subsequent ion-exchange adsorption was used for the recuperation of the clavulanic acid of the top phase and purification getting a concentration factor of 2 and purification of 100% in relation to the amino acids lysine, histidine, proline and tyrosine. (AU)

• FAPESP's process: 05/55079-4 - Production and purification of clavulanic acid, cephamycin C and other bioactive metabolites of Streptomyces
• Grantee: Carlos Osamu Hokka
• Support Opportunities: Research Projects - Thematic Grants