Genotoxicity assessment of water soluble fractions of biodiesel and its diesel blends using the Salmonella assay and the in vitro MicroFlow (R) kit (Litron) assay

Author(s):Show less -

Leme, Daniela Morais ^[1] ; Grummt, Tamara ^[2] ; de Oliveira, Danielle Palma ^[3] ; Sehr, Andrea ^[2] ; Renz, Sylvia ^[2] ; Reinel, Sissy ^[2] ; Ferraz, Elisa R. A. ^[3] ; Rodrigues de Marchi, Mary Rosa ^[4] ; Machado, Marcos Canto ^[4] ; Zocolo, Guilherme Juliao ^[4] ; Marin-Morales, Maria Aparecida ^[1]

Affiliation

^[1] Univ Estadual Paulista UNESP, Dept Biol, BR-13506900 Rio Claro, SP - Brazil

^[2] Fed Environm Agcy, Bad Elster Branch, D-08645 Bad Elster - Germany

^[3] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[4] Univ Estadual Paulista UNESP, Inst Chem, BR-14800900 Araraquara, SP - Brazil

• Document type: Journal article
• Source: Chemosphere; v. 86, n. 5, p. 512-520, FEB 2012.
• Web of Science Citations: 15

Abstract

The designation of biodiesel as an environmental-friendly alternative to diesel oil has improved its commercialization and use. However, most biodiesel environmental safety studies refer to air pollution and so far there have been very few literature data about its impacts upon other biotic systems, e.g. water, and exposed organisms. Spill simulations in water were carried out with neat diesel and biodiesel and their blends aiming at assessing their genotoxic potentials should there be contaminations of water systems. The water soluble fractions (WSF) from the spill simulations were submitted to solid phase extraction with C-18 cartridge and the extracts obtained were evaluated carrying out genotoxic and mutagenic bioassays {[}the Salmonella assay and the in vitro MicroFlow (R) kit (Litron) assay]. Mutagenic and genotoxic effects were observed, respectively, in the Salmonella/microsome preincubation assay and the in vitro MN test carried out with the biodiesel WSF. This interesting result may be related to the presence of pollutants in biodiesel derived from the raw material source used in its production chain. The data showed that care while using biodiesel should be taken to avoid harmful effects on living organisms in cases of water pollution. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 07/04304-3 - EVALUATION OF GENOTOXIC AND MUTAGENIC EFFECTS OF COMMERCIAL BIODIESEL AND DIESEL BLENDS PURE AND IN SPILL SIMULATIONS IN WATER AND SOIL
• Grantee: Daniela Morais Leme
• Support Opportunities: Scholarships in Brazil - Doctorate