| Full text | |
| Author(s): |
Zanquetta, Melissa Moreira
[1]
;
Correa-Giannella, Maria Lucia
[1]
;
Giannella-Neto, Daniel
[2]
;
Alonso, Paulino Alberto
[3]
;
Martins Vaz Guimaraes, Ligia Maria
[3]
;
Meyer, Alberto
[3]
;
Ferreira Villares, Sandra Mara
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med, Lab Endocrinol Celular & Mol LIM 25, BR-01246903 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med, Lab Gastroenterol Clin & Expt LIM 07, BR-01246903 Sao Paulo - Brazil
[3] Complexo Hosp Edmundo Vasconcelos, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | CHRONOBIOLOGY INTERNATIONAL; v. 29, n. 3, p. 252-260, 2012. |
| Web of Science Citations: | 5 |
| Abstract | |
Disrupted circadian rhythms are associated with obesity and metabolic alterations, but little is known about the participation of peripheral circadian clock machinery in these processes. The aim of the present study was to analyze RNA expression of clock genes in subcutaneous (SAT) and visceral (VAT) adipose tissues of male and female subjects in AM (morning) and PM (afternoon) periods, and its interactions with body mass index (BMI). Ninety-one subjects (41 +/- 11 yrs of age) presenting a wide range of BMI (21.4 to 48.6 kg/m(2)) were included. SAT and VAT biopsies were obtained from patients undergoing abdominal surgeries. Clock genes expressions were evaluated by qRT-PCR. The only clock gene that showed higher expression (p < .0001) in SAT in comparison to VAT was PER1 of female (372%) and male (326%) subjects. Different patterns of expression between the AM and PM periods were observed, in particular REV-ERBa, which was reduced (p < .05) at the PM period in SAT and VAT of both women and men (women: similar to 53% lower; men: similar to 78% lower), whereas CLOCK expression was not altered. Relationships between clock genes were different in SAT vs. VAT. BMI was negatively correlated with SATPER1 (r = -.549; p = .001) and SATPER2 (r = -.613; p = .0001) and positively with VATCLOCK (r = .541; p = .001) and VATBMAL1 (r = .468; p = .007) only in women. These data suggest that the circadian clock machinery of adipose tissue depots differs between female and male subjects, with a sex-specific effect observed for some genes. BMI correlated with clock genes, but at this moment it is not possible to establish the cause-effect relationship. (Author correspondence: mzanquetta@gmail.com) (AU) | |
| FAPESP's process: | 09/03065-0 - Study of genes related to circadian rhythm and GLUT-4 gene in obese people |
| Grantee: | Sandra Mara Ferreira Villares |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 08/05601-4 - Study of genes related to circadian rhythm and GLUT-4 gene in obese people |
| Grantee: | Melissa Moreira Zanquetta |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |