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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)


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Taxini, C. L. [1] ; Takakura, A. C. [2] ; Gargaglioni, L. H. [1] ; Moreira, T. S. [3]
Total Authors: 4
[1] Sao Paulo State Univ UNESP, Dept Morphol & Anim Physiol, BR-14884900 Jaboticabal, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508900 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Neuroscience; v. 199, p. 177-186, DEC 29 2011.
Web of Science Citations: 17

Central chemoreflex stimulation produces an increase in phrenic nerve activity (PNA) and sympathetic nerve activity (SNA). The A5 noradrenergic region projects to several brainstem areas involved in autonomic regulation and contributes to the increase in SNA elicited by peripheral chemoreflex activation. The aim of the present study was to further test the hypothesis that the A5 noradrenergic region could contribute to central chemoreflex activation. In urethane-anesthetized, sino-aortic denervated, and vagotomized male Wistar rats (n=6-8/group), hypercapnia (end-expiratory CO(2) from 5% to 10%) increased mean arterial pressure (MAP; Delta= +33+/-4 mmHg, P<0.05), splanchnic SNA (sSNA; Delta=+97+/-13%, P<0.05), and PNA frequency and amplitude. Bilateral injection of muscimol (GABA-A agonist; 2 mM) into the A5 noradrenergic region reduced the rise in MAP (Delta=+19+/-3 mmHg, P<0.05), sSNA (Delta= +63+/-5%, P<0.05), and PNA frequency and amplitude produced by hypercapnia. Injections of the immunotoxin anti-dopamine beta-hydroxylasesaporin (anti-D beta H-SAP) into the A5 region destroyed TH(+) neurons but spared facial motoneurons and the chemosensitive neurons in the retrotrapezoid nucleus that express the transcription factor Phox2b and that are non-catecholaminergic (TH(-)Phox2b(+)). Two weeks after selective destruction of the A5 region with the anti-DIM-SAP toxin, the increase in MAP (Delta=+22+/-5 mmHg, P<0.05), sSNA (Delta=+68+/-9%, P<0.05), and PNA amplitude was reduced after central chemoreflex activation. These results suggest that A5 noradrenergic neurons contribute to the increase in MAP, sSNA, and PNA activation during central chemoreflex stimulation. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 10/15501-7 - Role of A5 noradrenergic neurons in the cardiorespiratory response to hypoxia and hypercapnia
Grantee:Luciane Helena Gargaglioni Batalhão
Support type: Regular Research Grants
FAPESP's process: 10/09776-3 - Neural mechanisms involved in expiratory rhythm generator: possible involvement of the retrotrapezoid nucleus and the parafacial region
Grantee:Ana Carolina Thomaz Takakura
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 10/19336-0 - Pontine mechanisms involved in cardiorespiratory control during central or peripheral chemoreceptors activation
Grantee:Thiago dos Santos Moreira
Support type: Regular Research Grants