Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Lobe Variation Effects of Experimental Diabetes and Insulin Replacement on Rat Prostate

Full text
Author(s):
Porto, Elaine Manoela [1, 2] ; De Alcantara Dos Santos, Sergio Alexandre [3] ; Ribeiro, Larissa Mayumi [3] ; Lacorte, Livia Maria [3] ; Rinaldi, Jaqueline Carvalho [3] ; Justulin, Jr., Luis Antonio [4] ; Felisbino, Sergio Luis [3]
Total Authors: 7
Affiliation:
[1] State Univ W Parana UNIOESTE, Ctr Biol & Hlth Sci CCBS, Cascavel, Parana - Brazil
[2] Univ Campinas UNICAMP, Inst Biol, Sao Paulo - Brazil
[3] Univ Estadual Paulista UNESP, Dept Morphol, Inst Biosci, BR-18618000 Botucatu, SP - Brazil
[4] Univ Fed Triangulo Mineiro, Uberaba, MG - Brazil
Total Affiliations: 4
Document type: Journal article
Source: MICROSCOPY RESEARCH AND TECHNIQUE; v. 74, n. 11, p. 1040-1048, NOV 2011.
Web of Science Citations: 9
Abstract

We investigated the impact of diabetes with simultaneous and late insulin replacement on rat prostate growth during puberty, paying special attention to different prostatic lobes. Diabetes was induced by administration of streptozotocin (STZ) in 40-day-old male Wistar rats. A subset of diabetic rats underwent simultaneous insulin replacement (3 days after STZ administration), and another subset underwent a late insulin replacement (20 days after STZ administration). The ventral, dorsolateral, and anterior prostatic lobes were weighed and processed for histological, immunohistochemical, and morphometric analyses. Both diabetic and insulin-treated animals maintained low plasma testosterone (T) concentrations, whereas dihydrotestostenore (DHT) levels were normal. Diabetic animals had a decreased gain in absolute prostatic weight when compared to age-matched controls and insulin replacement animals. However, prostatic lobe weight in the diabetic animals was similar to 100% higher, even at the beginning of the experiment. Among the lobes, the anterior lobe showed the highest weight gain in diabetic and insulin replacement conditions. Epithelial cell proliferation in all lobes was significantly reduced in diabetic animals and significantly increased in insulin replacement animals, although apoptosis was unaltered. In conclusion, diabetes diminishes, but does not abolish, prostate growth during puberty. Even late insulin administration reduces the adverse effects of this disease on the prostate. In a scenario with both low insulin and T levels, DHT and other factors may play an important role in pubertal prostate growth. The adverse effects of diabetes on the rat prostate show a variation in lobe response, suggesting that diabetes may affect human prostate zones differently. Microsc. Res. Tech. 74:1040-1048, 2011. (C) 2011 Wiley Periodicals, Inc. (AU)