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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of toxic effects of a diet containing fish contaminated with methylmercury in rats mimicking the exposure in the Amazon riverside population

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Author(s):
Grotto, Denise [1] ; Valentini, Juliana [1] ; Serpeloni, Juliana Mara [1] ; Ponte Monteiro, Patricia Alves [2] ; Latorraca, Elder Francisco [3] ; de Oliveira, Ricardo Santos [3] ; Greggi Antunes, Lusania Maria [1] ; Garcia, Solange Cristina [4] ; Barbosa, Jr., Fernando [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Patol, BR-14040903 Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Cirurgia & Anat, BR-14040903 Sao Paulo - Brazil
[4] Univ Fed Rio Grande do Sul, Dept Anal Clin, BR-90046900 Porto Alegre, RS - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Environmental Research; v. 111, n. 8, p. 1074-1082, NOV 2011.
Web of Science Citations: 17
Abstract

This study was designed to evaluate the effects of a diet rich in fish contaminated with MeHg, mimicking the typical diet of the Amazon riverside population, in rats. Animals were randomly assigned to one of three groups with eight rats in each group: Group I-control, received commercial ration: Group II-received a diet rich in uncontaminated fish; Group III-received a diet rich in fish contaminated with MeHg. Treatment time was 12 weeks. Oxidative stress markers were evaluated, as well as the effects of this diet on DNA stability, systolic blood pressure (SBP), nitric oxide (NO) levels and histological damage in different tissues. There was a significant increase in SBP values in rats fed with MeHg-contaminated fish diet after the 10th week of the treatment. As far as oxidative stress biomarkers are concerned, no differences were observed in reduced glutathione and protein carbonyl levels, glutathione peroxidase, catalase, superoxide dismutase or delta-aminolevulinate dehydratase activities between the groups of animals receiving contaminated and uncontaminated fish diets. On the other hand, malondialdehyde levels increased significantly in rats fed with contaminated fish. NO levels were similar in all groups. DNA migration showed augmented in rats exposed to contaminated fish and histopathological analyses showed weak but significant leukocyte infiltration. Thus, we conclude that the MeHg-contaminated fish diet induced a slight lipid peroxidation and genotoxicity. However, these effects seem to be much less pronounced than when rats are exposed to aqueous solution containing CH(3)HgCl. Our findings support the contention that the chemical form of MeHg in fish or fish nutrients such as polyunsaturated fatty acids, Se or vitamin E could minimize the toxic effects of MeHg exposure in fish-eating communities. (C) 2011 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 11/07416-2 - Evaluation of sample preparation procedures for Arsenic speciation in biological samples by LC-ICP-MS
Grantee:Fernando Barbosa Júnior
Support type: Regular Research Grants
FAPESP's process: 11/07498-9 - Proteomic analysis in brain and kidney of rats exposed to different forms of mercury
Grantee:Fernando Barbosa Júnior
Support type: Regular Research Grants
FAPESP's process: 09/11102-3 - Chemical speciation of selenium and mercury in biological samples for toxicological and/or nutrional evaluation
Grantee:Fernando Barbosa Júnior
Support type: Regular Research Grants
FAPESP's process: 07/05221-4 - Evaluation of oxidative stress and nitric oxide availability in rats exposed to fish contaminated to MeHg
Grantee:Denise Grotto
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 07/04538-4 - Gene expression profile in pharmacoresistant epilepsy in children
Grantee:Helio Rubens Machado
Support type: Regular Research Grants