A comparison between different reference genes for expression studies in human hippocampal tissue

Author(s)

Maurer-Morelli, Claudia V. ; de Vasconcellos, Jaira F. ; Reis-Pinto, Fernanda C. ; Rocha, Cristiane de S. ; Domingues, Romenia R. ; Yasuda, Clarissa L. ^[1] ; Tedeschi, Helder ^[1] ; De Oliveira, Evandro ^[1] ; Cendes, Fernando ^[1] ; Lopes-Cendes, Iscia ^[2]

Affiliation

^[1] Univ Campinas UNICAMP, Dept Neurol, BR-13083887 Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Fac Med Sci, Dept Med Genet, BR-13083887 Campinas, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF NEUROSCIENCE METHODS; v. 208, n. 1, p. 44-47, JUN 30 2012.
• Web of Science Citations: 8

Abstract

The reliability of gene expression studies by mRNA quantification is highly dependent upon several experimental procedures, including the choice of reference genes used for data normalization. In order to contribute to gene expression studies in mesial temporal lobe epilepsy (MTLE) we used microarray data, followed by real time quantitative PCR validation of selected housekeeping genes, to determine the most appropriate reference genes to be used in human hippocampal tissue gene expression studies. Our results unequivocally showed a significant impact of the reference gene chosen for normalization on the overall results of expression studies, clearly demonstrating the importance of adequate validation using stable reference genes. In addition, we found that HPRT, NSE, SDHA and SYP are suitable genes to be used as reference for normalization in expression studies of hippocampal tissue obtained from patients with MTLE. (C) 2012 Published by Elsevier B.V (AU)

• FAPESP's process: 05/56578-4 - Multimodal neuroimaging center for studies in epilepsy
• Grantee: Fernando Cendes
• Support type: Inter-institutional Cooperation in Support of Brain Research (CINAPCE) - Thematic Grants