Microchip electrophoresis with amperometric detection for the study of the generation of nitric oxide by NONOate salts

Author(s)

Gunasekara, Dulan B. ^{[1, 2]} ; Hulvey, Matthew K. ^{[1, 3]} ; Lunte, Susan M. ^{[1, 2, 4]} ; Fracassi da Silva, Jose Alberto ^{[5, 1]}

Affiliation

^[1] Univ Kansas, Ralph N Adams Inst Bioanalyt Chem, Lawrence, KS 66045 - USA

^[2] Univ Kansas, Dept Chem, Lawrence, KS 66045 - USA

^[3] Akermin Inc, St Louis, MO 63134 - USA

^[4] Univ Kansas, Dept Pharmaceut Chem, Lawrence, KS 66045 - USA

^[5] Univ Estadual Campinas, Inst Quim, BR-13083000 Campinas, SP - Brazil

• Document type: Journal article
• Source: ANALYTICAL AND BIOANALYTICAL CHEMISTRY; v. 403, n. 8, p. 2377-2384, JUN 2012.
• Web of Science Citations: 16

Abstract

Microchip electrophoresis (ME) with electrochemical detection was used to monitor nitric oxide (NO) production from diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) and 1-(hydroxyl-NNO-azoxy)-l-proline disodium salt (PROLI/NO). NO was generated through acid hydrolysis of these NONOate salts. The products of acid hydrolysis were introduced into a 5-cm separation channel using gated injection. The separation was accomplished using reverse polarity and a background electrolyte consisting of 10 mM boric acid and 2 mM tetradecyltrimethylammonium bromide, pH 11. Electrochemical detection was performed using an isolated potentiostat in an in-channel configuration. Potentials applied to the working electrode, typically higher than +1.0 V vs. Ag/AgCl, allowed the direct detection of nitrite, NO, DEA/NO, and PROLI/NO. Baseline resolution was achieved for the separation of PROLI/NO and NO while resolution between DEA/NO and NO was poor (1.0 +/- 0.2). Nitrite was present in all samples tested. (AU)

• FAPESP's process: 10/01046-6 - Development of microfluidic devices with electrochemical detection for in vitro single cell analysis of nitrogen reactive species
• Grantee: José Alberto Fracassi da Silva
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 08/57805-2 - Institute of Bioanalytics
• Grantee: Lauro Tatsuo Kubota
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 08/53868-0 - Synthesis and chacterization of novel materials strategically structured and organized to be applied in analytical and photonic devices
• Grantee: Lauro Tatsuo Kubota
• Support Opportunities: Research Projects - Thematic Grants