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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Correlation between Clinical/Radiographic Features and Inflammatory Cytokine Networks Produced by Macrophages Stimulated with Endodontic Content

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Author(s):
Martinho, Frederico C. [1] ; Chiesa, Wanderson M. M. [1] ; Leite, Fabio R. M. [2] ; Cirelli, Joni A. [3] ; Gomes, Brenda P. F. A. [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Endodont Div, Piracicaba, SP - Brazil
[2] Univ Fed Pelotas, Sch Dent, Periodont Div, Dept Semiol & Clin, Pelotas, Rio Grande Do S - Brazil
[3] State Univ Sao Paulo, Araraquara Dent Sch, Periodont Div, Dept Diag & Oral Surg, Araraquara, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF ENDODONTICS; v. 38, n. 6, p. 740-745, JUN 2012.
Web of Science Citations: 41
Abstract

Introduction: Macrophages are highly activated by endodontic contents. This study investigated the correlation between different clinical signs/symptoms and radiographic features according to the levels of interleukin (IL)-1 beta, tumor necrosis factor alpha (TNF-alpha), IL-6, IL-10, prostaglandin E-2 (PGE(2)), and their networks produced by endodontic content-stimulated macrophages collected from primary endodontic infection with apical periodontitis (PEIAP). Methods: Samples were taken from 21 root canals with PEIAP by using paper points. The presence of exudate (EX), pain on palpation (POP), tenderness to percussion (UP), and the size of the radiographic lesion (SRL) were recorded. Polymerase chain reaction (16S rDNA) was used for bacterial detection and limulus amebocyte lysate (LAL) assay for endotoxin measurement. Raw 264.7 macrophages were stimulated with bacterial contents during 24 hs. The amounts of IL-1 beta, TNF-alpha, IL-6, IL-10 and PGE(2) were measured by enzyme-linked immunosorbent assay. Log-based data were correlated by multiple logistic regression (P < .05). Results: Bacteria and endotoxin were detected in 100% of the samples. IL-6 and TNF-alpha were positively correlated with SRL and EX, respectively (P < .05). Clinical signs/symptoms and radiographic findings were set as dependent variables for EX-positive correlations between PGE(2), IL-1 beta, and TNF-alpha (P < .05), whereas IL-6 and PGE(2) were positively correlated to each other in POP but negatively correlated in SRL (P < .05). When POP and TTP-POP were set as dependent variables, different cytokine networks were found. Conclusions: Our findings suggest different roles for each cytokine in the development of apical periodontitis, whose effects of overlapping networks depend on the signs/symptoms and radiographic features found in endodontic infection. (J Endod 2012;38:740-745) (AU)

FAPESP's process: 10/17877-4 - Microbiological analysis and quantification of endotoxins and proinflammatory cytokines from primarily infected root canals with apical periodontitis
Grantee:Brenda Paula Figueiredo de Almeida Gomes
Support Opportunities: Regular Research Grants
FAPESP's process: 10/19136-1 - Microbiological and endotoxins analysis in primarily infected root canals with apical periodontitis and their antigenic potencial against macrophages to produce IL1-beta e TNF-alfa during endodontic treatment
Grantee:Brenda Paula Figueiredo de Almeida Gomes
Support Opportunities: Regular Research Grants