Highly Sensitive Neurotransmitters Analysis at Platinum-Ultramicroelectrodes Arrays

Author(s)

Moraes, Fernando C. ^[1] ; Cesarino, Ivana ^[1] ; Coelho, Dyovani ^[1] ; Pedrosa, Valber A. ^[2] ; Machado, Sergio A. S. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, Sao Carlos, SP - Brazil

^[2] UNESP, Dept Chem & Biochem, Inst Biosci, Botucatu, SP - Brazil

• Document type: Journal article
• Source: Electroanalysis; v. 24, n. 5, p. 1115-1120, MAY 2012.
• Web of Science Citations: 3

Abstract

An easy way to determine norepinephrine (NE) in biological fluid using a platinum ultramicroelectrode array (Pt-UMEAs) is described. Issues related to UME electrode surface treatment and characterizations are also addressed. At optimized experimental conditions the dynamic concentration range was 1.0 to 10.0 mu mol?L-1 with a detection limit of 40.5 nmol?L-1. The repeatability of current responses for injections of 5 mu mol?L-1 NE was evaluated to be 4.0?% (n=10). This approach obtained excellent sensitivity, a reliable calibration profile and stable electrochemical response for norepinephrine detection. The content of NE in urine samples without any preconcentration, purification, or pretreatment step, was successfully analyzed by the standard addition method using the Pt-UMEAs. (AU)

• FAPESP's process: 10/11049-2 - Development and applications of electrochemical sensors based on aligned carbon nanotubes for endocrine disrupting detection in environmental samples
• Grantee: Fernando Cruz de Moraes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 10/07478-5 - Chain reaction amplified biocatalytic responses in nanostructured plasmonic sensors
• Grantee: Valber de Albuquerque Pedrosa
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 10/11567-3 - On-site analysis of the products formed in the electrochemical degradation of monoaromatic hydrocarbons
• Grantee: Ivana Cesarino
• Support Opportunities: Scholarships in Brazil - Post-Doctoral