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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Expression profile of malignant and non-malignant diseases of the thyroid gland reveals altered expression of a common set of genes in goiter and papillary carcinomas

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Stolf, Beatriz S. ; Abreu, Cintia M. ; Mahler-Araújo, Maria B. ; Dellamano, Márcia ; Martins, Waleska K. ; Carvalho, Marcos Brasilino de ; Curado, Maria P. ; Díaz, Juan P. ; Fabri, Artur ; Brentani, Helena ; et al
Total Authors: 11
Document type: Journal article
Source: Cancer Letters; v. 227, n. 1, p. 59-73, Sept. 2005.
Field of knowledge: Health Sciences - Medicine
Abstract

Using cDNA microarrays with 3800 cDNA fragments, we determined the expression profile of normal thyroid tissue, goiter, adenoma and papillary carcinoma (10 samples from each class). After background correction and statistical analysis, we identified a set of 160 genes as being differentially expressed in all pair-wise comparisons. Here we demonstrate that, at least on the basis of these differentially expressed genes, a positive correlation between goiter and papillary carcinomas could be observed. We identified a common set of genes whose expression is diminished in both goiter and papillary carcinomas as compared to normal thyroid tissue. Moreover, no genes with inverse correlation in samples from goiter and papillary carcinomas could be detected. Using Real-Time PCR and/or tissue microarrays, we confirmed the altered expression of some of the identified genes. Of notice, we demonstrate that the reduced mRNA levels of p27kip1 observed in papillary carcinomas as compared to either goiter or normal thyroid tissues (P<0.001) is accompanied by an altered protein distribution within the cell. In papillary carcinomas, P27KIP1 is preferentially cytoplasmic as opposed to goiter or normal thyroid tissue, where P27KIP1 is preferentially located in the nucleus. The exploitation of the data presented here could contribute to the understanding of the molecular events related to thyroid diseases and gives support to the notion that common molecular events might be related to the frequent observation of areas of papillary carcinomas in the gland of patients with goiter. (AU)

FAPESP's process: 98/14335-2 - Antonio Prudente Cancer Research Center
Grantee:Fernando Augusto Soares
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC