| Author(s): |
da Silva, Marcia R. M.
[1]
;
Uyhara, Cibele N. S.
[1]
;
Silva, Flavio H.
[2]
;
Espindola, Noeli M.
[3]
;
Poleti, Mirele D.
[1]
;
Vaz, Adelaide J.
[3]
;
Meirelles, Flavio V.
[1]
;
Maia, Antonio A. M.
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, FZEA, Dept Ciencias Basicas, BR-13635900 Pirassununga, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-13565905 Sao Carlos, SP - Brazil
[3] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 3
|
Silva M. R. M., Uyhara C.N.S., Silva F. H., Espindola N.M., Poleti M. D., Vaz A.J., Meirelles F. V. \& Maia A. A. M. 2012. Cysticercosis in experimentally and naturally infected pigs: Parasitological and immunological diagnosis. Pesquisa Veterinaria Brasileira 32(4): 297-302. Departamento de Ciencias Basicas, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de Sao Paulo, Av. Duque de Caxias Norte 225, Pirassununga, SP 13635-900, Brazil. E-mail: antomaia@usp.br. Our objective was to evaluate the diagnosis of swine cysticercosis by examining ``ante mortem{''} (inspection of the tongue), ``post mortem{''} (inspection and detailed necropsy) and ELISA for research in serum of antibodies (Ab-ELISA) and antigens (Ag-ELISA). Seven (7) pigs were experimentally infected orally with eggs of Taenia solium and another 10 were naturally infected. In the pigs experimentally infected, inspection of the tongue was negative in all animals, in the routine inspection detailed necropsy and cysticercis were identified in all of them. In pigs with heavy natural infection, inspection of the tongue identified cysticerci in two (20%), while at inspection with necropsy the parasites were identified in large quantities in all animals. In ELISA for antibody search (Ab-ELISA) TS-14 recombinant protein was used, and in search for antigen (Ag-ELISA) a monoclonal antibody against this protein. In animals experimentally infected, blood was collected weekly for 140 days. The Ab-ELISA identified an increase in titers of antibody to cysticerci 21 days after infection, and at the end of the experimental period six animals (86%) were positive to the test. The search for circulating antigens (Ag-ELISA) was positive in two pigs 28 to 91 days after infection. All naturally infected pigs were positive for Ag-ELISA and Ab-ELISA. The search for antibodies and antigens by ELISA in serum from 30 pigs of a local farm and without history of cysticercosis was negative. Thus, the use of TS-14 antigen in ELISA test (Ab-ELISA) can be useful for the diagnosis of cysticercosis in pigs with low infection. (AU) |