miR-15a and 16-1 Are Downregulated in CD4(+) T Cells of Multiple Sclerosis Relapsing Patients

Author(s)

Cetrulo Lorenzi, Julio Cesar ^{[1, 2]} ; Brum, Doralina G. ^[3] ; Zanette, Dalila L. ^{[1, 2]} ; Alves Souza, Alessandra de Paula ^{[2, 4]} ; Barbuzano, Fernanda Goncalves ^[2] ; dos Santos, Antonio Carlos ^[5] ; Barreira, Amilton Antunes ^[3] ; Silva, Jr., Wilson Araujo ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Dept Genet, Med Sch Ribeirao Preto, BR-14049 Ribeirao Preto - Brazil

^[2] Natl Inst Sci & Technol Stem Cell & Cell Therapy, Ribeirao Preto - Brazil

^[3] Univ Sao Paulo, Dept Neurosci, Med Sch Ribeirao Preto, BR-14049 Ribeirao Preto - Brazil

^[4] Univ Sao Paulo, Dept Immunol, Med Sch Ribeirao Preto, BR-14049 Ribeirao Preto - Brazil

^[5] Univ Sao Paulo, Dept Internal Med, Med Sch Ribeirao Preto, BR-14049 Ribeirao Preto - Brazil

• Document type: Journal article
• Source: International Journal of Neuroscience; v. 122, n. 8, p. 466-471, AUG 2012.
• Web of Science Citations: 32

Abstract

The pathology of relapsing-remitting multiple sclerosis (RR-MS) is largely attributed to activated autoreactive effector T lymphocytes. The influence of microRNAs on the immune response has been shown to occur in different pathways of lymphocyte differentiation and function. Here, the expression of the miRNAs miR-15a/161 in PBMC, CD4(+), and CD8(+) from RR-MS patients has been investigated. BCL2, a known miR-15a/16-1 target, has also been analyzed. The results have shown that miR-15a/16-1 is downregulated in CD4(+) T cells, whereas BCL2 is highly expressed in RR-MS patients only. Our data suggest that miR-15a/16-1 can also modulate the BCL2 gene expression in CD4(+) T cells from RR-MS patients, thereby affecting apoptosis processes. (AU)

• FAPESP's process: 98/14247-6 - Center for Research on Cell-Based Therapy
• Grantee: Marco Antonio Zago
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 10/00310-1 - miRNA signature of T lymphocyte subpopulations from healthy and multiple sclerosis patients
• Grantee: Julio Cesar Cetrulo Lorenzi
• Support Opportunities: Scholarships in Brazil - Doctorate