| Full text | |
| Author(s): |
da Silva Nali, Luiz Henrique
[1]
;
Centrone, Cristiane de Campos
[1]
;
Palma Urbano, Paulo Roberto
[1]
;
Penalva-De-Oliveira, Augusto Cesar
[2]
;
Vidal, Jose Ernesto
[2]
;
Miranda, Erique Peixoto
[3]
;
Pannuti, Claudio Sergio
[1]
;
Domingues da Silva Fink, Maria Cristina
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Lab Virol LIM HC FMUSP, Inst Med Trop Sao Paulo, BR-05403000 Sao Paulo - Brazil
[2] Inst Infectol Emilio Ribas, Sao Paulo - Brazil
[3] Univ Sao Paulo, Dept Molestias Infecciosas & Parasitarias, Fac Med, BR-05403000 Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Revista do Instituto de Medicina Tropical de São Paulo; v. 54, n. 4, p. 201-205, JUL-AUG 2012. |
| Web of Science Citations: | 13 |
| Abstract | |
Objective: To evaluate the prevalence of the urinary excretion of BKV and JCV in HIV-infected patients without neurological symptoms. Methods: Urine samples from HIV-infected patients without neurological symptoms were tested for JC virus and BK virus by PCR. Samples were screened for the presence of polyomavirus with sets of primers complementary to the early region of JCV and BKV genome (AgT). The presence of JC virus or BK virus were confirmed by two other PCR assays using sets of primers complementary to the VP1 gene of each virus. Analysis of the data was performed by the Kruskal-Wallis test for numerical data and Pearson or Yates for categorical variables. Results: A total of 75 patients were included in the study. The overall prevalence of polyomavirus DNA urinary shedding was 67/75 (89.3%). Only BKV DNA was detected in 14/75 (18.7%) urine samples, and only JCV DNA was detected in 11/75 (14.7%) samples. Both BKV and JCV DNA were present in 42/75 (56.0%) samples. Conclusion: In this study we found high rates of excretion of JCV, BKV, and simultaneous excretion in HIV+ patients. Also these results differ from the others available on the literature. (AU) | |
| FAPESP's process: | 07/04681-1 - Molecular characterization of human polyomavirus JC from patients with AIDS, with and without progressive multifocal leukoencephalopathy in São Paulo, Brazil |
| Grantee: | Claudio Sergio Pannuti |
| Support Opportunities: | Regular Research Grants |