Biocompatible Magnetic Microspheres for Use in PDT and Hyperthermia

Author(s)

Vaccari, C. B. ^[1] ; Cerize, N. N. P. ^[2] ; Morais, P. C. ^[3] ; Re, M. I. ^[2] ; Tedesco, A. C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ctr Nanotecnol & Engn Tecidual, Dept Quim, Lab Fotobiol & Fotomed, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] IPT Inst Techonol Res Sao Paulo State, Ctr Proc & Prod Technol, Sao Paulo - Brazil

^[3] Univ Brasilia, Inst Fis, BR-70910900 Brasilia, DF - Brazil

• Document type: Journal article
• Source: Journal of Nanoscience and Nanotechnology; v. 12, n. 6, p. 5111-5116, JUN 2012.
• Web of Science Citations: 9

Abstract

Loaded microspheres with a silicon (IV) phthalocyanine derivative (NzPC) acting as a photosensitizer were prepared from polyhydroxybutyrate-co-valerate (PHBHV) and poly(ecaprolactone) (PCL) polymers using the emulsification solvent evaporation method (EE). The aim of our study was to prepare two systems of these biodegradable PHBHV/PCL microspheres. The first one containing only photosensitizer previously incorporated in the PHBHV and poly(ecaprolactone) (PCL) microspheres and the second one with the post magnetization of the DDS with magnetic nanoparticles. Magnetic fluid is successfully used for controlled incorporation of nanosized magnetic particles within the micron-sized template. This is the first time that we could get a successful pos incorporation of nanosized magnetic particles in a previously-prepared polymeric template. This procedure opens a great number of possibilities of post-functionalization of polymeric micro or nanoparticles with different bioactive materials. The NzPC release profile of the systems is ideal for PDT, the zeta potential and the size particle are stable upon aging in time. In vitro studies were evaluated using gingival fibroblastic cell line. The dark citotoxicity, the phototoxicity and the AC magnetic field assays of the as-prepared nanomagnetic composite were evaluated and the cellular viability analyzed by the classical test of MU. (AU)

• FAPESP's process: 06/57129-1 - Estudos de micro esferas de PHBHV/PCL como sistemas biodegradáveis de liberação de fármaco para terapia fotodinâmica (TFD)
• Grantee: Carolina Bortolatti Vaccari
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 08/53719-4 - Use of nanocarriers containing photosensitive dyes and other derived and growing factors applied to the photodynamic process in the health areas
• Grantee: Antonio Claudio Tedesco
• Support Opportunities: Research Projects - Thematic Grants