Inflammatory milieu as an early marker of kidney injury in offspring rats from diabetic mothers

Author(s)

Correa-Costa, Matheus ; Landgraf, Maristella A. ; Cavanal, Maria F. ^[1] ; Semedo, Patricia ^[2] ; Vieira, Daniel A. G. ^[1] ; De Marco, Davi T. K. ^[1] ; Hirata, Aparecida E. ^[1] ; Camara, Niels O. S. ^{[3, 2]} ; Gil, Frida Z. ^[1]

Affiliation

^[1] Univ Fed Sao Paulo, Dept Physiol, BR-04039000 Sao Paulo - Brazil

^[2] Fed Univ Sao Paulo UNIFESP, Div Nephrol, BR-04039001 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci 4, Dept Immunol, Lab Transplantat Immunobiol, BR-05508000 Sao Paulo - Brazil

• Document type: Journal article
• Source: European Journal of Pharmacology; v. 689, n. 1-3, p. 233-240, AUG 15 2012.
• Web of Science Citations: 6

Abstract

The present study investigated the early presence of inflammatory response in renal tissue of young offspring from diabetic mothers. The effect of L-arginine (L-arg) supplementation was also investigated. The offspring was divided into four groups: group CO (controls); group DO (diabetic offspring); group CA (CO receiving 2% L-arg solution) and group DA (DO receiving the 2% L-arg solution). Glycemia, arterial pressure and renal function were evaluated; gene and protein expression of pro-inflammatory cytokines were also measured. Blood pressure levels were significantly increased in 2 and 6 month-old DO rats, whereas L-arg administration caused a significant decrease in the DA group, at both ages. DO rats showed a significantly blunted glycemic response to exogenous insulin. In 2 month-old DO animals, renal protein expression of pro-inflammatory molecules was significantly increased. At six months of age, we also observed an increase in gene expression of pro-inflammatory molecules, whereas L-arg supplementation prevented this increase at both ages. Our data suggest that activation of inflammatory pathways is present early in the kidney of DO rats, and that L-arg can attenuate the expression of these markers of tissue inflammation. Our results also reinforce the concept that intrauterine environmental factors are a fundamental determinant in the development of metabolic and vascular diseases later in life. (C) 2012 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 07/07139-3 - The role of heme oxygenase 1 in different renal inflammatory process in experimental animal models
• Grantee: Niels Olsen Saraiva Câmara
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 10/17782-3 - A new model of hypertension: the offspring of diabetic mother-similarities to SHR and Dahl rats
• Grantee: Frida Zaladek Gil
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/02270-2 - New cellular, molecular and immunological mechanisms involved in acute and chronic renal injury: the search for new therapeutical approaches
• Grantee: Niels Olsen Saraiva Câmara
• Support Opportunities: Research Projects - Thematic Grants