| Full text | |
| Author(s): |
dos Santos, Camila Ramos
[1]
;
Paiva, Joice Helena
[1]
;
Meza, Andreia Navarro
[1]
;
Cota, Junio
[2]
;
Alvarez, Thabata Maria
[2]
;
Ruller, Roberto
[2]
;
Prade, Rolf Alexander
[3]
;
Squina, Fabio Marcio
[2]
;
Murakami, Mario Tyago
[1]
Total Authors: 9
|
| Affiliation: | [1] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Biociencias LNBio, BR-13083970 Campinas, SP - Brazil
[2] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, BR-13083970 Campinas, SP - Brazil
[3] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 - USA
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Journal of Structural Biology; v. 177, n. 2, p. 469-476, FEB 2012. |
| Web of Science Citations: | 18 |
| Abstract | |
The breakdown of beta-1,4-mannoside linkages in a variety of mannan-containing polysaccharides is of great importance in industrial processes such as kraft pulp delignification, food processing and production of second-generation biofuels, which puts a premium on studies regarding the prospection and engineering of beta-mannanases. In this work, a two-domain beta-mannanase from Thermotoga petrophila that encompasses a GH5 catalytic domain with a C-terminal CBM27 accessory domain, was functionally and structurally characterized. Kinetic and thermal denaturation experiments showed that the CBM27 domain provided thermo-protection to the catalytic domain, while no contribution on enzymatic activity was observed. The structure of the catalytic domain determined by SIRAS revealed a canonical (alpha/beta)(8)-barrel scaffold surrounded by loops and short helices that form the catalytic interface. Several structurally related ligand molecules interacting with TpMan were solved at high-resolution and resulted in a wide-range representation of the subsites forming the active-site cleft with residues W134, E198, R200, E235, H283 and W284 directly involved in glucose binding. (C) 2011 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 10/51890-8 - SMOLBnet 2.0: Structural studies of transcription factors involved in the regulation of hydrolytic enzyme genes and swollenin from Aspergillus niger and A. fumigatus |
| Grantee: | Mário Tyago Murakami |
| Support Opportunities: | Regular Research Grants |