MICROSATELLITE MARKERS FOR ASPIDOSPERMA POLYNEURON (APOCYNACEAE), AN ENDANGERED TROPICAL TREE SPECIES

Author(s)

Ferreira-Ramos, Ronai ^{[1, 2]} ; Monteiro, Mariza ^[3] ; Zucchi, Maria I. ^[4] ; Pinheiro, Jose B. ^[3] ; Martinez, Carlos A. ^[2] ; Mestriner, Moacyr A. ^[1] ; Alzate-Marin, Ana Lilia ^{[1, 5]}

Affiliation

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14049900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Programa Posgrad Biol Comparada, BR-14040901 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, BR-13400970 Sao Paulo - Brazil

^[4] Agencia Paulista Tecnol Agronegocios, BR-13400970 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Fac Med Ribeirao Preto, Programa Posgrad Genet, BR-14049900 Sao Paulo - Brazil

• Document type: Journal article
• Source: AMERICAN JOURNAL OF BOTANY; v. 98, n. 11, p. E300-E302, NOV 2011.
• Web of Science Citations: 4

Abstract

Premise of the study : This study was designed to develop and characterize a preliminary set of nuclear microsatellite markers for Aspidosperma polyneuron, an endangered tropical tree species. Methods and Results : Leaf samples were collected from a group of mature A. polyneuron trees in a large area along the Pardo and Mogi-Guacu river basins in Sao Paulo State in the southeastern region of Brazil. Twenty-five simple sequence repeat (SSR) markers were developed and 16 of 30 samples had polymorphic loci. The number of alleles per locus ranged from two to 17 (average = 7.06). A high diversity (H(e) = 0.65) and parentage exclusion probability for the first (0.9995) and second (0.9999) parents were measured. Conclusions : These 16 polymorphic markers suggest a high potential for genetic diversity, gene flow, and mating system analyses in A. polyneuron. (AU)

• FAPESP's process: 08/01559-3 - MATING SYSTEM, POLLEN FLOW AND GENETIC ISOLATION OF Aspidosperma polyneuron Müll. Arg. IN A FRAGMENT SITUATED IN SÃO PAULO STATE
• Grantee: Ronai Ferreira Ramos
• Support Opportunities: Scholarships in Brazil - Doctorate