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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Quantification of Listeria monocytogenes in minimally processed leafy vegetables using a combined method based on enrichment and 16S rRNA real-time PCR

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Author(s):
de Oliveira, Maria Aparecida [1] ; Abeid Ribeiro, Eliana Guimaraes [1] ; Morato Bergamini, Alzira Maria [1] ; Pereira De Martinis, Elaine Cristina [2]
Total Authors: 4
Affiliation:
[1] Lab Reg Ribeirao Preto, Inst Adolfo Lutz, BR-14085410 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: FOOD MICROBIOLOGY; v. 27, n. 1, p. 19-23, FEB 2010.
Web of Science Citations: 32
Abstract

Modern lifestyle markedly changed eating habits worldwide, with an increasing demand for ready-to-eat foods, such as minimally processed fruits and leafy greens. Packaging and storage conditions of those products may favor the growth of psychrotrophic bacteria, including the pathogen Listeria monocytogenes. In this work, minimally processed leafy vegetables samples (n = 162) from retail market from Ribeirao Preto, Sao Paulo, Brazil, were tested for the presence or absence of Listeria spp. by the immunoassay Listeria Rapid Test, Oxoid. Two L. monocytogenes positive and six artificially contaminated samples of minimally processed leafy vegetables were evaluated by the Most Probable Number (MPN) with detection by classical culture method and also culture method combined with real-time PCR (RTi-PCR) for 16S rRNA genes of L monocytogenes. Positive MPN enrichment tubes were analyzed by RTi-PCR with primers specific for L. monocytogenes using the commercial preparation ABSOLUTET (TM) QPCR SYBR (R) Green Mix (ABgene, UK). Real-time PCR assay presented good exclusivity and inclusivity results and no statistical significant difference was found in comparison with the conventional culture method (p < 0.05). Moreover, RTi-PCR was fist and easy to perform, with MPN results obtained in ca. 48 h for RTi-PCR in comparison to 7 days for conventional method. (C) 2009 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 06/06401-3 - PCR targeting 16S rRNA genes for speeding enumeration of Listeria monocytogenes in minimally processed vegetables compared to the classical method
Grantee:Elaine Cristina Pereira de Martinis
Support Opportunities: Regular Research Grants