An evaluation of cell proliferation and adhesion on vertically-aligned multi-walled carbon nanotube films

Author(s)

Lobo, A. O. ^{[1, 2]} ; Corat, M. A. F. ^[3] ; Antunes, E. F. ^{[1, 2]} ; Palma, M. B. S. ^{[4, 2]} ; Pacheco-Soares, C. ^[4] ; Garcia, E. E. ^[5] ; Corat, E. J. ^{[1, 2]}

Affiliation

^[1] Inst Tecnol Aeronaut CTA, BR-12228900 Sao Jose Dos Campos, SP - Brazil

^[2] Inst Nacl Pesquisas Espaciais, Lab Assoc Sensores & Mat, BR-12245970 Sao Jose Dos Campos, SP - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Ctr Multidisciplinar Invest Biol, Area Ciencia Anim Lab CEMIB, BR-13083877 Campinas, SP - Brazil

^[4] Univ Vale Paraiba, Inst Pesquisa & Desenvolvimento, Lab Dinam Compartimentos Celulares, BR-12227010 Sao Jose Dos Campos, SP - Brazil

^[5] Univ Estadual Maringa, Dept Quim, BR-87020090 Maringa, Parana - Brazil

• Document type: Journal article
• Source: Carbon; v. 48, n. 1, p. 245-254, JAN 2010.
• Web of Science Citations: 42

Abstract

Biocompatibility tests were performed on vertically-aligned multi-walled carbon nanotube (MWCNT) films produced by microwave plasma chemical vapor deposition on titanium substrates with iron (Fe) and nickel (Ni) as catalysts. The cell adhesion and morphology of L-929 mouse fibroblast cells were studied by high resolution scanning electron microscopy, after up to 7 days incubation periods. Cell viability and proliferation were evaluated by two ``in vitro{''} tests: (1) 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide (MTT), and (2) lactate dehydrogenase (LDH) assays. Low level of bioavailable Fe and Ni was determined by inductively coupled plasma optical emission spectrometry. Neither functionalization nor purification of MWCNT films was necessary to obtain good response to the biocompatibility tests. Efficient cell growth and non-toxicity suggest the use MWCNTs in tissue regeneration. The MWCNT films stimulated the cell growth, showing a proliferation 20% higher than on Ti. (C) 2009 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 07/00013-4 - New materials, studies and applications of CVD diamond, diamond-like-carbon (DLC) and nanostructured carbon obtained by chemical vapor deposition
• Grantee: Evaldo Jose Corat
• Support Opportunities: Research Projects - Thematic Grants