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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Histomorphology and ultrastructure of pancreatic islet tissue during in vivo maturation of rat pancreas

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Author(s):
Carvalho, Carolina Prado de França [1] ; Martins, Junia Carolina Rebelo ; Cunha, Daniel Andrade da ; Boschero, Antonio Carlos [4] ; Collares-Buzato, Carla Beatriz [5]
Total Authors: 5
Affiliation:
[1] Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia - Brasil
[4] Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia - Brasil
[5] Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia - Brasil
Total Affiliations: 5
Document type: Journal article
Source: ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER; v. 188, n. 3, p. 221-234, May 2006.
Field of knowledge: Biological Sciences - Physiology
Abstract

In this study, we have investigated the structural and ultrastructural features of pancreatic islet tissue during rat postnatal development. For this purpose, we used neonatal (1-2 days old), young (21 days old) and adult (3-4 months old) rats. From a functional point of view, neonatal islet tissue displayed a relatively poor insulin secretory response to glucose stimulation in comparison with the adult ones. Histological analysis showed that neonatal islet cells display a less organized morphology in comparison with the young and adult ones, characterized by a less defined form and the presence of ductal structures within or nearby the islet. Regarding the islet cytoarchitecture, no differences were observed among all animal groups studied. B-cells were always typically detected within the islet core while A-cells occupied the islet periphery area. No marked differences were found during postnatal animal development regarding the ultrastructural aspect of the endocrine cells and their secretory granules. Nevertheless, quantitative analysis showed a lower B-cell/non-B-cell ratio, a higher association with ducts and an increased immunoreaction for proliferating cell nuclear antigen (PCNA) in neonatal islets as compared to young and adults. In conclusion, the acquisition of an adult pattern of insulin secretion may require an appropriate histoarchitecture and B-cell/non-B-cell proportion that may affect crucial regulatory events such as the paracrine and/or the cell-cell interaction or communication within the islet. (AU)

FAPESP's process: 02/13218-0 - Insulin secretion by pancreatic B-cells during pregnancy and the perinatal period of life. Participation of prolactin and insulin signaling cascades in the secretory process and alterations induced by protein malnutrition and disturbances of lipid metabolism
Grantee:Antonio Carlos Boschiero
Support Opportunities: Research Projects - Thematic Grants