Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Carbonyl stress and a combination of astaxanthin/vitamin C induce biochemical changes in human neutrophils

Full text
Author(s):
Guerra, B. A. [1] ; Bolin, A. P. [1] ; Otton, R. [1]
Total Authors: 3
Affiliation:
[1] Univ Cruzeiro Sul, Postgrad Program, CBS, BR-03342000 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: TOXICOLOGY IN VITRO; v. 26, n. 7, p. 1181-1190, OCT 2012.
Web of Science Citations: 5
Abstract

The purpose of the present study was to find out whether co-treatment of human neutrophils with high glucose and methylglyoxal (MGO) can alter the biochemical parameters of human neutrophils. We also examined if astaxanthin associated with vitamin C can improve those biochemical parameters. Neutrophils from healthy subjects were treated with 20 mM of glucose and 30 mu M MGO followed or not by the addition of the antioxidants astaxanthin (2 mu M) and vitamin C (100 mu M). MGO/high glucose treatment reduced the phagocytic capacity and the G6PDH, total/SOD and GR activities. Additionally, there was an increase in the activity of myeloperoxidase (MPO) with consequent increase in the hypochlorous acid production, CAT activity and in the release of IL-6 cytokine without changes in intracellular calcium mobilization. Our study also shows that the association of astaxanthin with vitamin C greatly improved neutrophil phagocytic capacity, decreasing all reactive oxygen species measured, pro-inflammatory IL-1 beta and TNF-alpha release, MPO activity and HCIO production. The combination of astaxanthin with vitamin C alone has more antioxidant and anti-inflammatory effects than when they were in the presence of MGO/high glucose. Injury to the function of neutrophils due to high glucose and methylglyoxal appears not to involve oxidative stress or calcium release. The association of antioxidants astaxanthin and vitamin C promoted a significant improvement in the function of neutrophils and in the redox status. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 09/14382-7 - Effect of methylglyoxal and glucose on the function of human leukocytes: possible protective role of astaxantin and vitamin C
Grantee:Rosemari Otton
Support Opportunities: Regular Research Grants
FAPESP's process: 09/17381-1 - Addition of glucose and methylglyoxal on neutrophil function: role of astaxanthin and vitamin C
Grantee:Beatriz Alves Guerra
Support Opportunities: Scholarships in Brazil - Scientific Initiation