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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Spectrophotometric evaluation of the behavior of disperse red 1 dye in aqueous media and its interaction with calf thymus ds-DNA

Full text
Author(s):
Uliana, Carolina V. [1] ; Garbellini, Gustavo S. [1] ; Yamanaka, Hideko [1]
Total Authors: 3
Affiliation:
[1] Univ Estadual Paulista UNESP, Inst Quim, BR-14800900 Araraquara, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 23, n. 8, p. 1469-1475, AUG 2012.
Web of Science Citations: 5
Abstract

UV-Vis spectrophotometry was used to measure the solubility of disperse red 1 (DR1) dye in aqueous solutions, using different solvents and dispersants. The dye was quantified in water samples, and its interaction with calf thymus ds-DNA was investigated. The results showed that the commercial dispersant Fongranal® FB was suitable for the preparation of aqueous DR1 solutions. For the determination of DR1 in purified water, the limit of detection was 2.47 × 10-6 mol L-1, and the limit of quantification was 8.22 × 10-6 mol L-1. Percentage recoveries of 91.2 and 103% were obtained for DR1 concentrations of 3.00 × 10-6 and 40.0 × 10-6 mol L-1, respectively. The recoveries achieved for DR1 present in tap and river water were in the range 85.9-113%. The interaction of DR1 with calf thymus DNA was accompanied by hypochromic and hyperchromic effects, which were related to conformational changes and damage to the DNA double helix. (AU)

FAPESP's process: 08/10449-7 - Assessment of occurrence, toxicity/genotoxicity and degradation processes of dyes in effluents and surface water
Grantee:Maria Valnice Boldrin
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 08/08990-1 - Studies of DNA damage by textile dyes and the protective capacity of flavonoids using electrochemical biosensor
Grantee:Carolina Venturini Uliana
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 09/08161-8 - Evaluation of organophosphate pesticides and DNA interactions using electrochemical biosensor
Grantee:Gustavo Stoppa Garbellini
Support Opportunities: Scholarships in Brazil - Post-Doctoral