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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cytogenetic characterization reveals differences in nuclear organization of cystic cells among Brazilian species of Triatoma (Heteroptera, Reduviidae)

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Author(s):
Bardella, Vanessa Bellini [1] ; de Souza, Rosana Silistino [1] ; Barrera San Martin, Juca Abramo ; Tardeli de Jesus Andrade, Celia Guadalupe ; Laforga Vanzela, Andre Luis ; Vilela de Azeredo-Oliveira, Maria Tercilia [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista UNESP IBILCE, Dept Biol, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Cell Biology International; v. 36, n. 12, p. 1287-1291, DEC 2012.
Web of Science Citations: 1
Abstract

Cytogenetic studies in triatomines have described the occurrence of holokinetic chromosomes, heterochromatin distribution and the location of rDNA (ribosomal DNA) sites, but few aspects of nuclear organization in this group have been discussed. We have focused on ultrastructural and cytogenetic features and differences in cystic cells of seminiferous tubules between five species of Triatoma. Cystic cells showed evidence of polyploidy events and heterochromatic blocks appeared predominantly in the central region of the nuclei. Cytogenetic analyses showed that there was variation in chromocenter number between species, and that the central regions were AT-rich {[}DAPI(+) (4',6-diamidino-2-phenylindole(+))], whereas the periphery was CG-rich (CMA(+)). Another characteristic was the distribution of 45S rDNA, which differed according to the chromosomal location of this sequence. In all we have compared aspects of nuclear organization, polyploidy, heterochromatin, rDNA site distribution and methylation levels, as well as the relationships between five species of Triatoma from a cystic cell perspective. (AU)

FAPESP's process: 09/17816-8 - Cytogenetic study in triatomines of Triatoma, Panstrongylus and Rhodnius (Triatominae, Heteroptera) genus.
Grantee:Maria Tercília Vilela de Azeredo Oliveira
Support Opportunities: Regular Research Grants
FAPESP's process: 10/01193-9 - Morphological and ultrastructural spermatogenesis study, emphasizing the nucleolar cycle and the chromatoid body formation in triatomines (Triatominae, Heteroptera), Chagas disease vectors.
Grantee:Maria Tercília Vilela de Azeredo Oliveira
Support Opportunities: Regular Research Grants